This product is a 2X crystalized preparation. The minimum protein content is 80%, which is generally associated with purity. However, the purity of this material has not been determined.
P4762
Papain from papaya latex
lyophilized powder, ≥10 units/mg protein
Papain from papaya latex
Synonym(s):
Papainase
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Select a Size
25 MG
HUF 21,600.00
50 MG
HUF 29,700.00
100 MG
HUF 78,200.00
500 MG
HUF 205,000.00
1 G
HUF 307,000.00
5 G
HUF 1,040,000.00
About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
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Product Name
Papain from papaya latex, lyophilized powder, ≥10 units/mg protein
biological source
papaya (latex)
form
lyophilized powder
specific activity
≥10 units/mg protein
mol wt
23.4 kDa
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Quality Level
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1 of 4
This Item | P5306 | 76216 | 1495005 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - |
| biological source papaya (latex) | biological source papaya (latex) | biological source papaya | biological source - |
| specific activity ≥10 units/mg protein | specific activity ≥8.0 units/mg protein | specific activity ≥10 U/mg protein (~25 mg/ml) | specific activity - |
| form lyophilized powder | form lyophilized powder | form solution | form - |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) pharmaceutical (small molecule) |
| mol wt 23.4 kDa | mol wt 23.4 kDa | mol wt Mr ~23000 | mol wt - |
Application
Papain from papaya latex has been used:
- to digest carotid artery smooth muscle cells[1]
- in dissecting solutions
- to produce Fab fragments of antibodies[2]
- for cell dissociation since it has been shown to be more effective and less damaging with certain tissues[3][4]
- in the enzyme cocktail for dissociating human fetal retinal tissue[5]
- to digest chondrocytes/scaffold cultures for total DNA quantification for cell proliferation[6]
- as a supplement in neurobasal medium to produce retinal cell suspensions[7]
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
Biochem/physiol Actions
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Papain with wide specificity,[8] cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. Papain is considered to digest protein substrates more broadly than the pancreatic proteases.[9] Papain is commonly used in cell isolation procedures as it is highly efficient and less harmful than other proteases on certain tissues.[10] The use of papain is known to yield more viable smooth muscle cells, retaining the cell′s sensitivity to stimulants.[11] Papain papaya latex has antifungal activity against C. albicans and is a cysteine protease.
- The pH optimum is 6.0-7.0
- Papain hydrolyzes esters and amides
General description
Papain is a cysteine protease, found in Carica papaya. It is composed of a single polypeptide chain containing three disulfide bridges and a sulfhydryl group.[12]
Other Notes
One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.
View more information on papain at www.sigma-aldrich.com/enzymeexplorer.
Packaging
Package size based on protein content
Physical form
Lyophilized powder containing sodium acetate
Preparation Note
2× Crystallized
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Studies on Papaya Leaf Tea, 25-25 (2009)
Eco-Friendly Textile Dyeing and Finishing, 244-244 (2017)
Recent advances in chemoenzymatic peptide syntheses
Yazawa K and Numata K
Molecules (Basel), 19(9), 13755-13774 (2014)
PAPAIN ENZYME: A DIGESTIVE AID
PATEL T
International Journal of Clinical and Biomedical Research, 2(1), 52-53 (2016)
Immunopanning purification and long-term culture of human retinal ganglion cells
Zhang XM, et al.
Molecular Vision, 16(1), 2867-2867 (2010)
Articles
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Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
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QC Methods
Instructions
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This product is not tested for endotoxins. Currently, an endotoxin-tested version of Papain is not available.
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