S5171

Human Fibronectin

Name: Human Fibronectin
Brand: SIGMA
Price: 196000 HUF

formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture

Synonym(s):

sFN

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0.5 MG

HUF 196,000.00

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.75

MDL number:

MFCD00284706

Key Documents

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Product Name

Superfibronectin from human plasma, solution, BioReagent, suitable for cell culture

biological source

human

recombinant

expressed in E. coli

product line

BioReagent

form

solution

mol wt

~235-270 kDa

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1 μg/cm²

UniProt accession no.

P02751

shipped in

ambient

storage temp.

2-8°C

Quality Level

200

Gene Information

human ... FN1(2335)

Related Categories

Attachment Factors

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Molecular Biology & Functional Genomics

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Show Differences

1 of 4

This Item	F0895	F3542	ECM001
Sigma-Aldrich S5171 Superfibronectin from human plasma	Sigma-Aldrich F0895 FIBRONECTIN FROM HUMAN PLASMA	Sigma-Aldrich F3542 Fibronectin Fragment III₁-C human	Sigma-Aldrich ECM001 Fibronectin human
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
biological source human	biological source human plasma	biological source human	biological source human
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
mol wt ~235-270 kDa	mol wt -	mol wt 8-15 kDa	mol wt 259.5 kDa (The protein migrates as a higher band on SDS-PAGE due to glycosylation.)
form solution	form liquid	form lyophilized powder	form lyophilized powder
recombinant expressed in E. coli	recombinant -	recombinant expressed in E. coli	recombinant expressed in HEK 293 cells

Application

Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration.[1][2] Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.

Disclaimer

Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.

Other Notes

Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.

Preparation Note

As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Gentle cell handling with an ultra-slow instrument: creep-manipulation of cells

Thielecke H, et al.

Microscopica Acta, 11(11), 1230-1241 (2005)

A matrix form of fibronectin mediates enhanced binding of Streptococcus pyogenes to host tissue.

N Okada et al.

The Journal of biological chemistry, 272(43), 26978-26984 (1997-10-27)

The pathogenic Gram-positive bacterium Streptococcus pyogenes (group A streptococcus) binds to fibronectin via protein F. In this study, we have investigated the binding properties of protein F to various multimeric tissue forms of fibronectin that appear on cell surfaces and

Regulated GPCR trafficking to the plasma membrane: general issues and the CCR5 chemokine receptor example

Giornale di Psichiatria e di Neuropatologia (2012)

EGFR Expression in HER2-Driven Breast Cancer Cells.

Florian Weinberg et al.

International journal of molecular sciences, 21(23) (2020-12-03)

The epidermal growth factor receptor HER2 is overexpressed in 20% of breast cancer cases. HER2 is an orphan receptor that is activated ligand-independently by homodimerization. In addition, HER2 is able to heterodimerize with EGFR, HER3, and HER4. Heterodimerization has been

Superfibronectin is a functionally distinct form of fibronectin.

A Morla et al.

Nature, 367(6459), 193-196 (1994-01-13)

Fibronectin is an extracellular matrix protein that is important in development, wound healing and tumorigenesis. In the blood it is dimeric, but in tissues forms disulphide crosslinked fibrils. Here we show that a fragment from the first type-III repeat of

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