Guanidine hydrochloride solution

Name: Guanidine hydrochloride solution
Brand: SIGMA

BioUltra, ~8 M in H₂O

Synonym(s):

Aminoformamidine hydrochloride, Guanidinium chloride, Guanidium chloride

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About This Item

Empirical Formula (Hill Notation):

CH₅N₃ · HCl

CAS Number:

50-01-1

Molecular Weight:

95.53

UNSPSC Code:

12352107

NACRES:

NA.25

PubChem Substance ID:

57651286

MDL number:

MFCD00013026

Beilstein/REAXYS Number:

3591990

Key Documents

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Product Name

Guanidine hydrochloride solution, BioUltra, ~8 M in H₂O

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

SMILES string

Cl.NC(N)=N

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

product line

BioUltra

form

liquid

concentration

~8 M in H₂O

impurities

insoluble matter, passes filter test

refractive index

n20/D 1.465

pH

4.5-7.5 (25 °C)

anion traces

sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤10 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤5 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤150 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm A_max: 0.040
λ: 280 nm A_max: 0.025

Quality Level

100

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Biochemicals

Chemistry & Biochemicals

Detergents - Anionic, Cationic, Zwitterionic, Anti-foaming

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This Item	G9284	50940	G7294
Sigma-Aldrich 50937 Guanidine hydrochloride solution	Sigma-Aldrich G9284 Guanidine hydrochloride solution	Sigma-Aldrich 50940 Guanidine hydrochloride	Sigma-Aldrich G7294 Guanidine hydrochloride solution
concentration ~8 M in H₂O	concentration -	concentration -	concentration 8 M
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 200
product line BioUltra	product line -	product line -	product line -
form liquid	form liquid	form solid	form buffered aqueous solution
pH 4.5-7.5 (25 °C)	pH -	pH (25 °C, 4.6 - 6/573 g/L)	pH 8.5
cation traces Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Ca: ≤10 mg/kg, Co: ≤1 mg/kg, Cu: ≤1 mg/kg, K: ≤20 mg/kg, Mg: ≤5 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Sr: ≤1 mg/kg, As: ≤0.1 mg/kg, Cd: ≤1 mg/kg, Fe: ≤1 mg/kg, Mn: ≤1 mg/kg, Pb: ≤1 mg/kg, Bi: ≤1 mg/kg, Li: ≤1 mg/kg, Zn: ≤1 mg/kg, Cr: ≤1 mg/kg, Na: ≤150 mg/kg	cation traces -	cation traces Ca: ≤20 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg	cation traces -

Application

Guanidine hydrochloride solution has been used as a fixation buffer in genomic DNA extraction for genomic analysis.[1]

Biochem/physiol Actions

Guanidine‐HCl (Gnd-HCl), an efficient chaotropic agent, is broadly used in RNA isolation, globular protein denaturation, and protein refolding studies as a denaturant. It is an appropriate sample buffer for the quick and effective elution of affinity-purified protein complexes and the subsequent protein digestion step.[2] Gnd-HCl is not preferred in proteomics experiments due to its hindrance with trypsin activity at low molar concentrations and tendency to precipitate when combined with sodium dodecyl sulfate (SDS).[2] GnHCl is commonly utilized to extract proteins in extracellular matrix (ECM)‐rich tissues including bone, cartilage, ligament, and tendon.[3]

pictograms

GHS07

signalword

Warning

hcodes

H302 + H332,H315,H319

pcodes

P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Class III Peroxidases in Response to Multiple Abiotic Stresses in Arabidopsis thaliana Pyrenean Populations

Eljebbawi A, et al.

International Journal of Molecular Sciences (2022)

Comparison between chaotropic and detergent-based sample preparation workflow in tendon for mass spectrometry analysis.

Yalda Ashraf Kharaz et al.

Proteomics, 17(13-14) (2017-05-27)

Exploring the tendon proteome is a challenging but important task for understanding the mechanisms of physiological/pathological processes during ageing and disease and for the development of new treatments. Several extraction methods have been utilised for tendon mass spectrometry, however different

Using guanidine-hydrochloride for fast and efficient protein digestion and single-step affinity-purification mass spectrometry.

Jon W Poulsen et al.

Journal of proteome research, 12(2), 1020-1030 (2012-11-29)

Protein digestion is an integral part of the "shotgun" proteomics approach and commonly requires overnight incubation prior to mass spectrometry analysis. Quadruplicate "shotgun" proteomic analysis of whole yeast lysate demonstrated that Guanidine-Hydrochloride (Gnd-HCl) protein digestion can be optimally completed within

Aminoalkyl derivatives of guanidine diaromatic minor groove binders with antiprotozoal activity.

Caitriona McKeever et al.

Journal of medicinal chemistry, 56(3), 700-711 (2013-01-11)

Considering the strong DNA minor groove binding observed for our previous series of diaromatic symmetric and asymmetric guanidinium and 2-aminoimidazolinium derivatives, we report now the synthesis of new aminoalkyl derivatives of diaromatic guanidines with potential as DNA minor groove binders

An improved synthesis of (-)-5,11-dideoxytetrodotoxin.

Masaatsu Adachi et al.

The Journal of organic chemistry, 78(4), 1699-1705 (2013-01-18)

We describe an improved synthesis of (-)-5,11-dideoxytetrodotoxin from an enone, which was used for synthesis of tetrodotoxin and its analogues in this laboratory. One of the major modifications was to establish a two-step guanidinylation of trichloroacetamide of a highly functionalized

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