07-2139
Anti-Cysteine Sulfenic Acid Antibody
serum, from rabbit
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この商品について
eCl@ss:
32160702
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
mouse, rat, human
Application:
WB
Citations:
2
この製品は、Merck Milliporeウェブサイトでのみ注文できます。
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
species reactivity (predicted by homology)
mammals
technique(s)
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
General description
Multiple kDa are observed that increases with oxidized stress (H2O2).
Protein sulfenic acid formation is a reversible post-translational modification that may be used to monitor protein oxidation on reactive cysteines within target proteins. This can be detected with protein sulfenic acid derivatised with dimedone.
Immunogen
Epitope: Other
KLH-conjugated corresponding to cysteine sulfenic acid.
Application
Anti-Cysteine Sulfenic Acid detects levels of Cysteine Sulfenic Acid proteins & has been published & validated for use in WB.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Western Blot Analysis: A previous lot of this antibody detected cysteine sulfenic acid in NIH/3T3 and HEK293 cell lysates.
Biochem/physiol Actions
This antibody recognizes proteins containing cysteine sulfenic acid derivitized with dimedone.
Physical form
Unpurified
Unpurified rabbit polyclonal with 0.05% sodium azide.
Preparation Note
Stable for 1 year at from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Rat ventricular myocyte lysate
Rat ventricular myocyte lysate
Evaluated by Western Blot in rat ventricular myocyte lysate.
Western Blot Analysis: 1:10,000 dilution of this antibody detected cysteine sulfenic acid on 10 µg of rat ventricular myocyte lysate.
Western Blot Analysis: 1:10,000 dilution of this antibody detected cysteine sulfenic acid on 10 µg of rat ventricular myocyte lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
07-2139:
jan