The difference between the Type I and Type II Asialofetuin products does not represent any kind of biochemical or functional difference. These indicate different methods of production and purification. The Type II option, product A1908, utilizes neuraminadase in an enzymatic cleavage step. The Type I, product A4781, utilizes a proprietary modification of that described in Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).
A1908
アシアロフェツイン ウシ胎児血清由来
Type II (Sigma designation)
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| サイズ/SKU | 在庫状況 | 単価 |
|---|---|---|
50 mg | カートの在庫状況を確認する | ￥122,000 |
この商品について
Form:
powder
Biological source:
bovine
biological source
bovine
Quality Segment
type
Type II (Sigma designation)
form
powder
technique(s)
activity assay: suitable, inhibition assay: suitable
impurities
salt, essentially free, ≤1.0% N-acetylneuraminic acid
solubility
H2O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow
storage temp.
2-8°C
Application
Asialofetuin from fetal calf serum is suitable for use:
リポソームにおけるアシアロフェチュイン(AF)の取り込みは、AF受容体を提示している細胞、すなわち肝細胞への送達および肝細胞によるエンドサイトーシスを強力に亢進します。これは、遺伝子療法のきわめて効率的な経路となります。
Biochem/physiol Actions
Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain. It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.
Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.
Preparation Note
Neuraminidaseを用いて酵素的に切断
1 of 1
当該品目 | |||
|---|---|---|---|
| description Type II (Sigma designation) | description Type I (Sigma designation) | description lyophilized powder, suitable for cell culture | description lyophilized powder |
| technique(s) inhibition assay: suitable, activity assay: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) HPLC: suitable, MALDI-MS: suitable |
| biological source bovine | biological source bovine (calf) serum | biological source bovine serum (fetal) | biological source bovine serum (fetal) |
| form powder | form powder | form lyophilized powder | form lyophilized powder |
| impurities ≤1.0% N-acetylneuraminic acid, salt, essentially free | impurities ≤0.5% N-acetylneuraminic acid, salt, essentially free | impurities ≤0.3% free N-acetylneuraminic acid | impurities ~0.2% free N-acetylneuraminic acid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility H2O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow | solubility 0.85% sodium chloride: soluble 1 mg/mL | solubility H2O: 1 mg/mL, colorless to faint yellow or tan | solubility - |
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保管分類
11 - Combustible Solids
一体何が起きているの？
WGK 3
引火点 (F)
Not applicable
引火点（°C）
Not applicable
PPE（個人用保護具）
Eyeshields, Gloves, type N95 (US)