This product is a liquid medium. The density is not reported. The 1L volume weighs approximately 1 kilogram. The total weight of the packaged 1L size is 1.1 kg. The total weight of the packaged 500 mL size is 578 g.
D6429
DMEM - high glucose
With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
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別名:
DME, DMEM
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| サイズ/SKU | 在庫状況 | 単価 |
|---|---|---|
500 mL | 在庫from市川市大町368 | ￥2,960 |
1 L | 在庫from市川市大町368 | ￥4,800 |
この商品について
UNSPSC Code:
12352207
NACRES:
NA.71
製品名
ダルベッコ改変イーグル培地(高グルコース), With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high
shipped in
ambient
storage temp.
2-8°C
General description
本製品は、ピルビン酸ナトリウム添加1x完全培地です。また、DMEM高グルコース組成とは異なり、ピリドキサールに代わりピリドキシンが使用されています。ピリドキサールは、培地の不安定成分の1つにあたります。
Application
ダルベッコ改変イーグル培地(DMEM)は、イーグル基礎培地(BME)を改変したもので、4倍濃度のアミノ酸とビタミンが含まれています。オリジナルの処方には、グルコース1000 mg/Lが含まれ、胚性マウス細胞の培養に使用されていました。それ以降、マウス、ニワトリの細胞のほか、様々な正常細胞やトランスフォーム細胞の初代培養をサポートするために幾通りかの方法で改変が行われてきました。これらの培地は、L-グルタミンとピルビン酸ナトリウムの組み合わせがそれぞれに異なります。また、グルコース濃度が4500 mg/Lまで引き上げられたことから、「DMEM/High」と命名されました。
1 of 1
当該品目 | |||
|---|---|---|---|
| description With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | description With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture | description With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture | description liquid, sterile-filtered, with 4500 mg/L dextrose, with 4.0 mM L-glutamine, without sodium pyruvate, suitable for cell culture |
| sterility sterile-filtered | sterility - | sterility - | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form powder | form powder | form liquid |
| components HEPES: no | components glucose: high | components phenol red: yes | components glucose: high |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
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保管分類
12 - Non Combustible Liquids
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How much does D6429-1L weigh? Thank you!
1 回答-
役に立ちましたか？
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Hi, What is the concentration of endotoxin detected in DMEM high glucose media D6429?
1 回答-
This product has an endotoxin specification of ≤ 1 EU/ml or 0.1 ng/mL. The lot specific values are reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d6429#product-documentation役に立ちましたか？
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What is the concentration of Sodium Bicarbonate and Sodium Pyruvate in it?
1 回答-
This formulation contains 3.7 g/L sodium bicarbonate and 110 mg/L sodium pyruvate. The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
役に立ちましたか？
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After opening a bottle of medium: how long can I use this bottle ?(stored at 4C°)
1 回答-
Provided that the product is handled and stored properly and has not been contaminated, the medium will be stable up to the expiration date. This date is provided on the label and the lot-specific Certificate of Analysis.
役に立ちましたか？
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I would like to inquire about the amino acid formulation in DMEM (#6429, #6546). Specifically, could you provide information on the concentration of aspartate and glutamate in these formulations? Thank you very much.
1 回答-
The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
役に立ちましたか？
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