The unit activity of this product is lot-specific and reported in the product Certificate of Analysis. The minimum activity is 250 units per microliter. The KU value represents 1000 units. For example, a 20 KU package size represents 20,000 units. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/e1014#product-documentation
E1014
Benzonase® ヌクレアーゼ
≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
別名:
エンドヌクレアーゼ Serratia marcescens(セラチア菌)由来
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About This Item
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詳細
アプリケーション
生物化学的/生理学的作用
特徴および利点
- マイクロバイオームサンプル中の宿主DNAの除去。
- さまざまなワークフローにおける効果的な核酸消化。
- タンパク質抽出時の粘度の低減。
単位の定義
物理的形状
法的情報
保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves
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試験成績書(COA)
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資料
Benzonase® Nuclease for reducing host DNA in microbiome workflows and enhancing taxa identification.
このページは、Benzonase®(ベンゾナーゼ)ヌクレアーゼについてよくある質問9項目とその回答をまとめたものです。
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
関連コンテンツ
The use of Benzonase® endonuclease can significantly reduce the levels of DNA by more than 100,000-fold while also reducing viscosity and protecting downstream equipment from DNA fouling. However, optimization strategies and DoE are critical when it comes to reducing DNA in your process. Setting up a DoE for your Benzonase® endonuclease application can help you find the optimal operation conditions that deliver the required DNA clearance from your process.
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How many ul does it contain? What kind of Unit is KU?
1 回答-
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Hi, for protein purification from E. coli cells, how much is the working concentration range for Benzonase nuclease (≥250 units/μL)? Thank you
1 回答-
The recommended starting concentration for Benzonase nuclease is 25 units per milliliter of cell lysate.
役に立ちましたか?
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What is the storage temperature range for E1014? There is only a specified storage temperature, and not a range.
1 回答-
This product is stored at freezer temperature, which is typically -20°C. An excepted range is -15 - -20°C.
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アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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