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F2012

抗マウスIgG (全分子)–FITC ヤギ宿主抗体

Name: 抗マウスIgG (全分子)–FITC ヤギ宿主抗体
Brand: SIGMA
Price: 22000 JPY

affinity isolated antibody, buffered aqueous solution

別名:

Goat Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate

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サイズ/SKU	在庫状況	単価
0.5 mL	カートの在庫状況を確認する	￥22,000
1 mL	カートの在庫状況を確認する	￥44,300
2 mL	カートの在庫状況を確認する	￥89,600

この商品について

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162642

Conjugate:

FITC conjugate

Clone:

polyclonal

Application:

IF (d)

Citations:

104

￥22,000

カートの在庫状況を確認する

特注のご依頼

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特性

biological source

goat

Quality Segment

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:64 (minimum)

storage temp.

2-8°C

target post-translational modification

unmodified

詳細

General description

全てのマウスイムノグロブリンに結合します。

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Application

Anti-Mouse IgG (whole molecule)−FITC antibody has been used:

in immunofluorescence
in immunohistochemistry
in immunofluorescence staining
in immunofluorescence microscopy
in confocal imaging

Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat was used for FACS analysis of DF3 antigen on breast cancer cell lines.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti-Mouse IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

Physical form

0.01M PBS溶液 (pH 7.4、1%BSA, 15mMアジ化ナトリウム含有)

Preparation Note

ヒトサンプルでのバックグラウンドの低減のため吸収処理済み。

Other Notes

本抗体は、ヒト血清タンパク質で吸収済です。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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当該品目	M4280	A3688	A0545
Sigma-Aldrich F2012 抗マウスIgG (全分子)–FITC ヤギ宿主抗体	Sigma-Aldrich M4280 抗マウスIgG (Fc特異的) ヤギ宿主抗体	Sigma-Aldrich A3688 抗マウスIgG (全分子)−アルカリフォスファターゼヤギ宿主抗体	Sigma-Aldrich A0545 抗ウサギIgG (全分子)-ペルオキシダーゼヤギ宿主抗体
description affinity isolated antibody, buffered aqueous solution	description 2.0 mg/mL, affinity isolated antibody	description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody
biological source goat	biological source goat	biological source goat	biological source goat
technique(s) direct immunofluorescence: 1:64 (minimum)	technique(s) indirect ELISA: 1:20,000	technique(s) dot blot: 1:30,000, direct ELISA: 1:30,000, western blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50	technique(s) western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate FITC conjugate	conjugate unconjugated	conjugate alkaline phosphatase conjugate	conjugate peroxidase conjugate
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
form buffered aqueous solution	form -	form buffered aqueous glycerol solution	form -

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保管分類

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c