F2012

抗マウスIgG (全分子)–FITC ヤギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: Goat Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162642
• Conjugate: FITC conjugate
• Clone: polyclonal
• Application: IF (d)
• Citations: 104

特性

• biological source: goat
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): direct immunofluorescence: 1:64 (minimum)
• storage temp.: 2-8°C
• target post-translational modification: unmodified

詳細

General description

全てのマウスイムノグロブリンに結合します。

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Application

Anti-Mouse IgG (whole molecule)−FITC antibody has been used:

• in immunofluorescence
• in immunohistochemistry
• in immunofluorescence staining
• in immunofluorescence microscopy
• in confocal imaging

Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat was used for FACS analysis of DF3 antigen on breast cancer cell lines.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti-Mouse IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

Physical form

0.01M PBS溶液 (pH 7.4、1%BSA, 15mMアジ化ナトリウム含有)

Preparation Note

ヒトサンプルでのバックグラウンドの低減のため吸収処理済み。

Other Notes

本抗体は、ヒト血清タンパク質で吸収済です。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• 一体何が起きているの？: nwg
• 引火点 (F): Not applicable
• 引火点（°C）: Not applicable