The Protease Colorimetric Detection Kit is designed for the accurate quantitation of protease activity. The method is based on the cleavage of casein into trichloroacetic acid-soluble peptides containing tyrosine and tryptophan residues. These peptides react with Folin-Ciocalteu reagent to give a characteristic blue color that can be measured at 660 nm.
Detection limit: 0.1 unit/ml (approx. 2 μg) trypsin with a 10 minute incubation time.
For greater sensitivity or for detection of trace protease activity, the Protease Fluorescent Detection kit (PF0100) is recommended.
Supplied with sufficient reagents to perform 200 one mL assays.
This kit has been tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).