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Merck

UFC5030

Amicon® Ultra Centrifugal Filter, 30 kDa MWCO

greener alternative

sample volume 0.5 mL, regenerated cellulose membrane, MWCO 30 kDa

동의어(들):

Centrifugal concentrator, 30 kDa MWCO, 0.5 mL sample volume


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크기 선택

24 PKG

₩222,894

96 PKG

₩791,802

500 PKG

₩3,286,063

₩222,894


예상 입고일2025년 12월 04일세부사항



제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
41104916
eCl@ss:
36100101
NACRES:
NB.24

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기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의

material

polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing

Quality Level

무균

non-sterile

제품 라인

Amicon®

포장

pkg of 24 pkg
pkg of 500 pkg
pkg of 8 pkg
pkg of 96 pkg

제조업체/상표

Amicon® Ultra

sustainability

Greener Alternative Product

파라미터

0.5 mL sample volume

기술

protein extraction: suitable
protein purification: suitable

길이

4.99 cm (1.96 in.)

직경

10.8 mm

여과 면적

1 cm2

용적

0.5 mL

작업 용적

≤500 μL
0.5 mL

공극 크기

30 kDa MWCO

환경친화적 대안 카테고리

배송 상태

ambient

유사한 품목 비교

전체 비교 보기

차이점 표시

1 of 4

이 품목
UFC203024UFC8030UFC9030
sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

material

polypropylene cap, regenerated cellulose membrane, polypropylene tube, styrene-butadiene housing

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

parameter

0.5 mL sample volume

parameter

2 mL sample volume

parameter

4 mL sample volume

parameter

15 mL sample volume

pore size

30 kDa MWCO

pore size

30 kDa MWCO

pore size

30 kDa MWCO

pore size

30 kDa MWCO

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

product line

Amicon®

product line

Amicon®

product line

Amicon®

product line

Amicon®

일반 설명

Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Ovalbumin (45 kDa) at 1 mg/ml initial concentration is typically retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

애플리케이션

  • Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
  • Desalting, buffer exchange, and protein dialysis

특징 및 장점

  • Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%
  • Concentrates 500 μL down to 15 μL
  • Concentration factor 25x to 30x
  • High sample recovery (greater than 90% of dilute starting solution)
  • Fast processing time: 10 to 30 minutes
  • Reverse spin capability provides consistent recoveries

물리적 형태

Color Code: Clear

기타 정보

Replaces: 42411

법적 정보

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.

Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.

나노입자 정제 및 생산에서 여과 방법 및 멤브레인 필터 선택.

모두 보기

프로토콜

Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.

If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.

질문

1–4 / 4 질문  
  1. What is the maximum Urea´s concentration that can resisten this Amicon Ultra 30 KDa?

    1 답변
    1. From the compatibility chart in the user guide, the devices can tolerate less than or equal to 8M urea.

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  2. What is the maximum speed of an Amicon Ultra 2 device?

    1 답변
    1. The maximum speeds for concentration spins as per the User Guide are as follows:
      • Swinging bucket rotor: 4,000 × g
      • Fixed angle rotor: 7,500 × g
      It is important to note that when spinning viscous solutions like undiluted serum or plasma, the maximum speed should not exceed 5,400 x g.

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  3. what is the pore sizes of 30kDa, 50kDA, and 100 kDa filters? And their corresponding maximum and minimum volume which can be used?

    1 답변
    1. These ultrafiltration do not have defined pore sizes. They are characterized by retention of globular proteins, and assigned nominal pore ratings. These MWCO membrane types are found in centrifugal devices with 70, 15, 4, 2, and 0.5 ml volume capacities.

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  4. Can I use Amicon® Ultra Centrifugal Filter, 30 kDa MWCO to measure human serum protein binding for a small moelcule?

    1 답변
    1. The Centrifree® Ultrafiltration Centrifugal Filters are recommended for this application over the Amicon® Ultra Centrifugal Filters. Please see the link below:
      https://www.sigmaaldrich.com/product/mm/4104

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