Merck
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83264

Sigma-Aldrich

HEPES buffer solution

1 M in H2O

Synonym(s):
HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
CAS Number:
Número MDL:
eCl@ss:
32129211
ID de la sustancia en PubChem:
NACRES:
NA.25

formulario

liquid

Nivel de calidad

concentración

1 M in H2O
238 g/L HEPES in H2O

impurezas

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.5±0.5

useful pH range

6.8-8.2

densidad

1.07 g/mL at 20 °C

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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H0887H3537H7523
HEPES buffer solution 1 M in H2O

Sigma-Aldrich

83264

HEPES buffer solution

HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

H0887

HEPES solution

HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Sigma-Aldrich

H3537

HEPES solution

HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sigma-Aldrich

H7523

HEPES

form

liquid

form

liquid

form

liquid

form

powder

concentration

1 M in H2O, 238 g/L HEPES in H2O

concentration

1 M

concentration

1 M

concentration

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

endotoxin, tested

impurities

Bioburden, tested, DNase, RNase, Protease, Nickase, free, endotoxin, tested

impurities

Insoluble matter, passes filter test

pH

5.5±0.5

pH

7.0-7.6

pH

5.0-6.0

pH

5.0-6.5 (1 M in H2O)

density

1.07 g/mL at 20 °C

density

-

density

-

density

-

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

HEPES has been used:
  • As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
  • To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
  • As a component of dissection buffer for culturing rat cortical neurons
  • Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
  • As a component of Danieu′s aquarium water used in agarose gel preparation

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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