CELLMM2

FLAG^® M Purification Kit

For Mammalian expression systems.

• Synonym(s): Anti-ddddk, Anti-dykddddk
• NACRES: NA.32

Properties

• Nivel de calidad: 200
• enviado en: wet ice
• temp. de almacenamiento: −20°C

Description

Envase

1 kit

Sufficient for 3-5 uses of 1-ml affinity purification column.

Aplicación

The FLAG Purification Kit utilizes CelLytic^™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG^® M2 affinity gel for affinity purification of active FLAG fusion proteins.

Browse additional application references in our FLAG^® Literature portal.

Características y beneficios

• Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
• ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
• Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).

Otras notas

Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.

Información legal

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

CelLytic is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• C2978 CelLytic^™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
• SAE0194 Purified 3xFLAG^® peptide, ≥95% (HPLC), lyophilized powder
• A2220 ANTI-FLAG^® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solution
• C2103 Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.

Safety Information

• Código de clase de almacenamiento: 10 - Combustible liquids
• WGK: WGK 3