SRP3014

BDNF human

Carrier free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), suitable for cell culture

• Sinónimos: Brain Derived Neurotrophic Factor
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: human
• Nivel de calidad: 200
• recombinante: expressed in E. coli
• Ensayo: ≥95% (SDS-PAGE)
• Formulario: lyophilized
• mol peso: 27.0 kDa
• envase: pkg of 10 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: ≤1.00 EU/μg endotoxin, tested
• Nº de acceso UniProt: P23560
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... BDNF(627)

Descripción

Descripción general

The gene BDNF (brain derived neurotrophic factor) is mapped to human chromosome 11p14.1.[1] BDNF is a member of the neurotrophin family of growth factors.[2][3] The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling.[4] BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0kDa homodimer of two 119 amino acid subunits linked by strong noncovalent interactions.

Acciones bioquímicas o fisiológicas

Like other members of this family, BDNF (brain derived neurotrophic factor) supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR (low-affinity nerve growth factor receptor), which also binds other neurotrophins such as NGF (nerve growth factor), NT-3 (neurotrophin-3) and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB (tropomyosin-related kinase B).[2] BDNF also plays an important role in vascular function and participates in angiogenesis.[5] It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus.[4]

Secuencia

MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Forma física

Lyophilized with no additives.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Nota de análisis

The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable