Merck

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
RESUMEN

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

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Sigma-Aldrich
N,N-Dimethylformamide, ACS reagent, ≥99.8%
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Methanol, ACS reagent, ≥99.8%
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Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
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Tetrahydrofuran, contains 250 ppm BHT as inhibitor, ACS reagent, ≥99.0%
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Chloroform, biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
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Methanol, puriss., meets analytical specification of Ph Eur, ≥99.7% (GC)
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Methanol, BioReagent, ≥99.93%
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Tetrahydrofuran, ACS reagent, ≥99.0%, contains 250 ppm BHT as inhibitor
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Chloroform, contains ethanol as stabilizer, ACS reagent, ≥99.8%
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Chloroform, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
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Chloroform, contains ethanol as stabilizer, meets analytical specification of BP, 99-99.4% (GC)
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Chloroform, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)
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1-Octanol, ACS reagent, ≥99%
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Chloroform, contains amylenes as stabilizer, ACS reagent, ≥99.8%
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Tetrahydrofuran, biotech. grade, ≥99.9%, Inhibitor-free
Sigma-Aldrich
N,N-Dimethylformamide, biotech. grade, ≥99.9%
USP
Diethylene glycol, United States Pharmacopeia (USP) Reference Standard
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Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
Supelco
Diethylene glycol, Pharmaceutical Secondary Standard; Certified Reference Material
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Formaldehyde solution, for molecular biology, 36.5-38% in H2O
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Sodium chloride solution, 5 M
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Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Supelco
Formaldehyde solution, stabilized with methanol, ~37 wt. % in H2O, certified reference material
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2-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
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Sodium chloride, BioXtra, ≥99.5% (AT)
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Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
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Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
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Sodium chloride, tablet
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Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
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Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer