M3148

2-Mercaptoethanol

for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

• Synonym(s): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
• Linear Formula: HSCH₂CH₂OH
• CAS Number: 60-24-2
• Molecular Weight: 78.13
• Beilstein: 773648
• Número de EC: 200-464-6
• Número MDL: MFCD00004890
• ID de la sustancia en PubChem: 24896800
• NACRES: NA.31

Properties

• grado: for molecular biology
• Nivel de calidad: 200
• densidad de vapor: 2.69 (vs air)
• presión de vapor: 1 mmHg ( 20 °C)
• Línea del producto: BioReagent
• ensayo: 99% (GC/titration)
• formulario: liquid
• lim. expl.: 18 %
• concentración: 14.3 M (pure liquid)
• technique(s): cell culture | mammalian: suitable; electrophoresis: suitable
• índice de refracción: n20/D 1.500 (lit.)
• pH: 4.5-6 (20 °C, 500 g/L)
• bp: 157 °C (lit.)
• solubilidad: H₂O: soluble 1 mL/mL
• densidad: 1.114 g/mL at 25 °C (lit.)
• actividad extraña: DNase, RNase, protease, none detected
• temp. de almacenamiento: room temp
• SMILES string: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

Aplicación

Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:

• as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
• to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
• to trypsinize mouse embryonic stem cells

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Envase

0.15, 0.6, 0.9, 2, 25, 100, 250, 500 mL in glass bottle

Safety Information

• pictogramas: GHS05,GHS06,GHS08,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H301 + H331 - H310 - H315 - H317 - H318 - H361d - H373 - H410
• Consejos de prudencia: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Código de clase de almacenamiento: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Punto de inflamabilidad F: 154.4 °F - closed cup
• Punto de inflamabilidad C: 68 °C - closed cup
• Equipo de protección personal: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter