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M3148

Sigma-Aldrich

2-Mercaptoethanol

for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Sinónimos:
β-Mercaptoethanol, BME, Thioethylene glycol, 2-Hydroxyethylmercaptan
Fórmula lineal:
HSCH2CH2OH
Número de CAS:
Peso molecular:
78.13
Beilstein:
773648
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.31

Nivel de calidad

200

grado

for molecular biology

densidad de vapor

2.69 (vs air)

presión de vapor

1 mmHg ( 20 °C)

línea de producto

BioReagent

ensayo

99% (GC/titration)

formulario

liquid

lim. expl.

18 %

concentración

14.3 M (pure liquid)

aplicaciones

cell culture | mammalian: suitable
electrophoresis: suitable

índice de refracción

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

solubilidad

H2O: soluble 1 mL/mL

densidad

1.114 g/mL at 25 °C (lit.)

actividad extraña

DNase, RNase, protease, none detected

temp. de almacenamiento

room temp

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

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Aplicación

Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:
  • as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
  • to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
  • to trypsinize mouse embryonic stem cells
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Envase

25, 100, 250, 500 mL in glass bottle

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

Código de clase de almacenamiento

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Punto de inflamabilidad F

154.4 °F - closed cup

Punto de inflamabilidad C

68 °C - closed cup

Equipo de protección personal

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificado de Análisis

Certificado de origen

A subset of platinum-containing chemotherapeutic agents kills cells by inducing ribosome biogenesis stress
Peter M Bruno
Nature Medicine, 23 (2017)
Reduced Innate Immune Response to a Staphylococcus aureus Small Colony Variant Compared to Its Wild-Type Parent Strain
Judy J
Frontiers in Cellular and Infection Microbiology, 6, 187-187 (2016)
Yasunari Seita et al.
Biology of reproduction, 100(6), 1440-1452 (2019-03-15)
Nonhuman primates (NHPs) are considered to be the most valuable models for human transgenic (Tg) research into disease because human pathology is more closely recapitulated in NHPs than rodents. Previous studies have reported the generation of Tg NHPs that ubiquitously
Kotaro Fujii et al.
Nature communications, 8, 14443-14443 (2017-02-15)
The degree and dynamics of translational control during mammalian development remain poorly understood. Here we monitored translation of the mammalian genome as cells become specified and organize into tissues in vivo. This identified unexpected and pervasive translational regulation of most
Manching Ku et al.
PLoS genetics, 4(10), e1000242-e1000242 (2008-11-01)
In embryonic stem (ES) cells, bivalent chromatin domains with overlapping repressive (H3 lysine 27 tri-methylation) and activating (H3 lysine 4 tri-methylation) histone modifications mark the promoters of more than 2,000 genes. To gain insight into the structure and function of

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