DNTP-RO

Deoxynucleoside Triphosphate Set

PCR Grade, sodium salt

• Synonim(y): datp, dctp, dgtp, dttp, set of datp, dctp, dgtp, and dttp, pcr grade
• NACRES: NA.21
• UNSPSC Code: 41106300
• Assay: 99% (dNTP)
• Form: liquid
• Solubility: water: miscible
• Storage temp.: −20°C

Właściwości

• grade: Molecular Biology
• assay: 99% (dNTP)
• form: liquid
• usage: sufficient for 37,500 reactions (03622614001), sufficient for 6,250 reactions (11969064001)
• packaging: pkg of 4 × 1,250 μL (03622614001 [4x100 mM]), pkg of 4 × 250 μL (11969064001 [4x100 mM])
• manufacturer/tradename: Roche
• concentration: <0.9% (dNDP)
• color: colorless
• solubility: water: miscible
• storage temp.: −20°C

Opis

General description

This product contains 4 separate vials of dATP, PCR Grade, dCTP, PCR Grade, dGTP, PCR Grade, and dTTP, PCR Grade, each with a concentration of 100 mM and as a clear, colorless solution of the sodium salt (pH 8.3).

Application

The Deoxynucleoside Triphosphate Set, PCR Grade, is suitable for many applications where high-quality reagents are required. Such applications include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.[1][2]

Features and Benefits

PCR Grade Nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.

• PCR Grade Nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
• They are provided as clear, colorless solutions in water at a concentration of 100 mM (pH 8.3).
• Due to the manufacturing process, PCR Grade Nucleotides are free of dNTPs with modified bases, as well as tetra- and pyrophosphate contamination. Tetraphosphates can disintegrate and form pyrophosphate, which is a known inhibitor of amplification reactions. Therefore, the purity of nucleotides is critical to the success of your amplification reactions.
• PCR Grade Nucleotides are stable for 30 months after the production date when stored at -15 to -25°C.

Packaging

1 set containing 4 components

• dATP, PCR Grade, sodium salt, 100 mM
• dCTP, PCR Grade, sodium salt, 100 mM
• dGTP, PCR Grade, sodium salt, 100 mM
• dTTP, PCR Grade, sodium salt, 100 mM

Preparation Note

Working solution: For best results, prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Analysis Note

Function tested using RT-PCR: Tested for absence of contaminating deoxyribonucleases, ribonucleases, and nicking activities according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Tylko elementy zestawu

• dATP, PCR Grade, sodium salt 100 mM
• dCTP, PCR Grade, sodium salt 100 mM
• dGTP, PCR Grade, sodium salt 100 mM
• dTTP, PCR Grade, sodium salt 100 mM

Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash