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X100

Triton™ X-100

laboratory grade

Synonim(y):

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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Rozmiar/SKUDostępnośćCena netto
5 mL

Dostępne do wysyłki DZISIAJzSchnelldorf Distribution

221,00 zł
100 mL

Dostępne do wysyłki DZISIAJzSchnelldorf Distribution

399,00 zł
500 mL

Dostępne do wysyłki DZISIAJzSchnelldorf Distribution

518,00 zł
1 L

Dostępne do wysyłki DZISIAJzSchnelldorf Distribution

714,00 zł
1 gal

Dostępne do wysyłki DZISIAJzSchnelldorf Distribution

2110,00 zł
5 gal

Przewidywany termin wysyłki03 lutego 2027zSchnelldorf Distribution

8200,00 zł

Informacje o tej pozycji

Wzór liniowy:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Numer CAS:
UNSPSC Code:
12161900
NACRES:
NA.21
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
2315025

221,00 zł


Dostępne do wysyłki DZISIAJSzczegóły


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biological source

synthetic

Quality Segment

grade

laboratory grade

description

non-ionic

form

liquid

mol wt

micellar avg mol wt 80,000, average mol wt 625

aggregation number

100-155

CMC

0.2-0.9 mM (20-25°C)

mp

6  °C (43 °F)

transition temp

cloud point 65 °C, pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

HLB

13.5

application(s)

advanced drug delivery

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

General description

Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Triton X-100 has hemolytic property and is used for DNA extraction.

Application

Triton™-X has been used:
  • In the permeabilization of cells for immunofluorescence staining
  • As a component of lysis buffer in western blot analysis
  • As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

  • Ideal for Cell Biology and Biochemical research applications
  • Non-ionic nondenaturing detergent
  • Superior wetting agent and emulsifier
  • Highly versatile surfactant

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Ta pozycja
T8787T9284X100PC
description

laboratory grade

description

Molecular Biology

description

BioXtra

description

peroxide- and carbonyl-free

form

liquid

form

liquid

form

liquid

form

liquid

grade

laboratory grade

grade

Molecular Biology

grade

-

grade

-

aggregation number

100-155

aggregation number

100-155

aggregation number

100-155

aggregation number

100-155

application(s)

advanced drug delivery

application(s)

hematology
histology

application(s)

advanced drug delivery

application(s)

-

mol wt

average mol wt 625, micellar avg mol wt 80,000

mol wt

micellar avg mol wt 80,000, average mol wt 625

mol wt

micellar avg mol wt 80,000, average mol wt 625

mol wt

micellar avg mol wt 80,000, average mol wt 625

mp

6  °C (43 °F)

mp

6 °C

mp

-

mp

-


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Słowo ostrzegawcze

Danger

Klasa składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

483.8 °F - closed cup

Temperatura zapłonu (°C)

251 °C - closed cup

PPE (środki ochrony indywidualnej)

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2


Wykazy regulacyjne

Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.

9036-19-5

CAS No.

9036-19-5

CAS No.


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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–10 of 20 Questions  
  1. Salve Questo è uno standard Certificato oppure un materiale di riferimento? Posso usarlo come standard per retta di taratura in HPLC_MS?

    1 answer
    1. This product is not a certified reference material, nor has it been tested for use in HPLC/MS. As Triton X-100 is typically avoided in Mass Spectrometry, there is no analytical standard currently that is qualified for this application. The end user would have to determine suitability for use in HPLC/MS. However, this material is referenced in an LC/MS publication by Shimadzu. Please see the link below to review:
      https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/technical/white_papers/13844/apo21759.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. What kind of change will be observed if heat up to 55C? Will it attack stacked Al and Ti metal layer? Can it mix with diluted nitric acid to etch Cu/NiV?

    1 answer
    1. Heating this product to 55°C may lead to changes in viscosity, solubility, or performance characteristics. There is no indication that this product can attack aluminum and titanium metal layers. This product is generally compatible with diluted nitric acid. While no specific incompatibility is reported, the combination may alter the properties of both the surfactant and the acid, potentially affecting the performance of either in applications such as cleaning or etching. Additionally, it is important to consider the concentration and the potential for foaming or other reactions during mixing.

      Helpful?

  4. Is this product nuclease-free?

    1 answer
    1. Helpful?

  5. How long is Triton X-100 stable at -80 degrees in buffer?

    1 answer
    1. At -80°C, Triton X-100 is generally stable, but the stability can depend on the specific buffer conditions and the concentration of the detergent. In most cases, Triton X-100 remains effective at low temperatures, but it is advisable to avoid repeated freeze-thaw cycles, as these can lead to changes in the detergent's properties and effectiveness. Please note that the shelf life after opening is dependent upon various factors which might affect the stability and, therefore, it is not guaranteed and needs to be determined by the end user.

      Helpful?

  6. If a sterilization of Triton X-100 or its dilutions (maximum 10% of Triton X-100 in the total volume) is needed to remove DNAse and RNAse, what's the best approach?

    1 answer
    1. Autoclaving undiluted Triton X-100 is generally not recommended for sterilization. However, dilute solutions, up to 10%, may be autoclaved. At certain
      concentrations, solutions may become cloudy, but dispersible above the cloud point. The solution will clarify with stirring upon cooling. Note that DNAse and RNAse-free products are currently available. Please see the links below to review these product options:
      https://www.sigmaaldrich.com/product/sigma/t8787
      https://www.sigmaaldrich.com/product/mm/648466
      https://www.sigmaaldrich.com/product/sigma/93443

      Helpful?

  7. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  8. What are the storage conditions for the Triton 100x? It's not in the COA

    1 answer
    1. This product may be be stored in room temperature.

      Helpful?

  9. Will heating Triton to reduce its viscosity have any negative impact on the product? Does heating negatively affect the activity of Triton?

    1 answer
    1. Triton X-100 can be frozen, thawed, and used. However, caution is advised when heating it above 50°C, as discoloration may occur. This note indicates that excessive heating can lead to some form of change in Triton X-100. It would be reasonable to assume that the recommended working range is from ambient temperature to 50°C (or slightly lower). If your process requires heating the solution to temperatures higher than 50°C, it is recommended to add Triton X-100 only after the temperature has dropped below 50°C or lower. If you need to work at temperatures exceeding 50°C, it is advisable to conduct solution stability studies to assess potential damage or breakdown of Triton X-100.

      Helpful?

  10. Is a concentration of 8x10^-5 mol/L of Triton X-100 harmful to metal (steel/aluminum) and silicon equipment when submerged in water?

    1 answer
    1. The laboratory has not specifically tested the corrosive properties of Triton X-100 on metal. However, according to a publication titled "Triton X-100 and Sodium Lauryl Sulfate Surfactants as Environmentally Friendly Corrosion Inhibitors for Steel in 0.5 M H2SO4" in Advances in Analytical Chemistry, Triton X-100 appears to act as a corrosion inhibitor for steel.

      Helpful?

1–10 of 20 Questions  

Reviews

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  1. Lillehammer, Norway
    • Reviews 2
    • Votes 5
    5 out of 5 stars.

    M.Sc.

    We use the Product for surface cleaning of artworks. Works as planned.

    Helpful?

  2. 1 Ratings-only review