Merck
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T8787

Sigma-Aldrich

Triton X-100

for molecular biology

Synonim(y):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Wzór liniowy:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Numer CAS:
Beilstein:
2315025
Numer MDL:
Identyfikator substancji w PubChem:
NACRES:
NA.21

Poziom jakości

200

klasa czystości

for molecular biology

opis

non-ionic

forma

liquid

masa cząsteczkowa

micellar avg mol wt 80,000
average mol wt 625

liczba agregacji

100-155

technique(s)

protein purification: suitable
protein quantification: suitable
western blot: suitable

pH

9.7

CMC

0.2-0.9 mM (20-25°C)

mp

6 °C

temp. przejścia

cloud point 65 °C
pour point ~7 °C

rozpuszczalność

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

gęstość

1.06 g/mL at 25 °C (lit.)

HLB

13.5

application(s)

diagnostic assay manufacturing
hematology
histology

działalność zagraniczna

DNase and RNase, none detected

temp. przechowywania

room temp

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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Opis ogólny

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Zastosowanie

Triton X-100 has been used:
  • In immunohistochemistry for staining the Flat-mount retinas
  • Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
  • To permeabilise cells during Immunofluorescent microscopic studies
  • As a positive control in LDH assay to determine the cell membrane integrity
  • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
  • For the preparation of outer membrane protein exctract
  • As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells
  • In the treatment of tissue sections for Immunofluorescence labeling

Opakowanie

50, 100, 250 mL in poly bottle

Działania biochem./fizjol.

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Informacje prawne

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Kod klasy składowania

10 - Combustible liquids

WGK

WGK 3

Temperatura zapłonu °F

483.8 °F

Temperatura zapłonu °C

251 °C

Środki ochrony indywidualnej

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certyfikat analizy

Wprowadź numer partii, aby wyszukać certyfikat analizy (COA).

Świadectwo pochodzenia

Wprowadź numer partii, aby wyszukać świadectwo pochodzenia (COO).

M A Ferguson et al.
Journal of applied physiology (Bethesda, Md. : 1985), 85(3), 1169-1174 (1998-09-08)
The purpose of this study was to determine the threshold of exercise energy expenditure necessary to change blood lipid and lipoprotein concentrations and lipoprotein lipase activity (LPLA) in healthy, trained men. On different days, 11 men (age, 26.7 +/- 6.1
Jean-Sébastien Joyal et al.
Blood, 117(22), 6024-6035 (2011-03-01)
The failure of blood vessels to revascularize ischemic neural tissue represents a significant challenge for vascular biology. Examples include proliferative retinopathies (PRs) such as retinopathy of prematurity and proliferative diabetic retinopathy, which are the leading causes of blindness in children
Effects of four different single exercise sessions on lipids, lipoproteins, and lipoprotein lipase.
Ferguson M A, et al.
Journal of Applied Physics, 85(3), 1169-1174 (1998)
Silver nanoparticle-induced cytotoxicity in rat brain endothelial cell culture.
Grosse S, et.
Toxicology in vitro, 27(1), 305-313 (2013)
Sealing the skin barrier around transcutaneous implants: in vitro study of keratinocyte proliferation and adhesion in response to surface modifications of titanium alloy.
Pendegrass C J, et al.
The Journal of Bone and Joint Surgery. British Volume, 90(1), 114-121 (2008)

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