71725

Sodium dodecyl sulfate

BioUltra, for molecular biology, ≥99.0% (GC)

• Synonym(s): Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
• Linear Formula: CH₃(CH₂)₁₁OSO₃Na
• CAS Number: 151-21-3
• Molecular Weight: 288.38
• Beilstein: 3599286
• Numer EC: 205-788-1
• Numer MDL: MFCD00036175
• eCl@ss: 39093306
• Identyfikator substancji w PubChem: 57652353
• NACRES: NA.25

Properties

• klasa czystości: for molecular biology
• Poziom jakości: 200
• opis: anionic
• linia produktu: BioUltra
• Próba: ≥99.0% (GC)
• forma: powder
• masa cząsteczkowa: micellar avg mol wt 18,000
• liczba agregacji: 62
• zanieczyszczenia: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• strata: ≤3% loss on drying, 20 °C (HV)
• CMC: 7-10 mM (20-25°C)
• mp: 204-207 °C (lit.)
• temp. przejścia: cloud point >100 °C
• rozpuszczalność: H₂O: 0.1 M at 20 °C, clear, colorless
• ślady anionów: chloride (Cl^-): ≤200 mg/kg; phosphate (PO₄^3-): ≤1 mg/kg
• ślady kationów: Al: ≤5 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤200 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.1 M in H₂O
• absorpcja UV: λ: 260 nm A_max: 0.04; λ: 280 nm A_max: 0.02
• SMILES string: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• HLB: 40
• przydatność: suitable for electrophoresis
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• InChI key: DBMJMQXJHONAFJ-UHFFFAOYSA-M

Description

Opis ogólny

Sodium dodecyl sulfate (SDS), an ionic detergent, is made up of a hydrophobic 12-carbon chain and a polar sulfate head group. This wetting agent is effective in both acid and alkaline solutions.

Zastosowanie

Sodium dodecyl sulfate has been used:

• as an ionic surfactant in polystyrene (PS) particle suspension for surfactant-based experiments
• in phosphate-buffered saline as a component of lysis buffer to homogenize myometrium and leiomyoma tissue
• as a component of lysis buffer to dissolve formazans
• as a component of high-salt nuclear extraction buffer

Anionic detergent

Działania biochem./fizjol.

Sodium dodecyl sulfate (SDS) serves as an important detergent in the structural biology field and protein folding/unfolding studies. The sulfate groups and alkyl chains of SDS can strongly bind to the positively charged and the hydrophobic residues of proteins. SDS induces the formation of α-helices in a protein structure. At high concentrations, SDS stimulates cooperative unfolding in various proteins such as bovine serum albumin (BSA), hen egg-white lysozyme, -lactalbumin, and -lactoglobulin structures.

Cechy i korzyści

• Undergoes hydrolysis at high temperatures, especially in acidic medium

Safety Information

• piktogramy: GHS02,GHS05,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H228 - H302 + H332 - H315 - H318 - H335 - H412
• Zwroty wskazujące środki ostrożności: P210 - P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 4.1B - Flammable solid hazardous materials
• WGK: WGK 2
• Temperatura zapłonu °F: 338.0 °F
• Temperatura zapłonu °C: 170 °C
• Środki ochrony indywidualnej: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges