Kluczowe dokumenty

Wyświetl całą dokumentację

Przejdź do

C1057

Butyrylcholinesterase from equine serum

Name: Butyrylcholinesterase from equine serum
Brand: SIGMA

lyophilized powder, ≥900 units/mg protein

Synonim(y):

Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase

Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.

Wybierz wielkość

Zmień widok

Rozmiar/SKU	Dostępność	Cena netto

Informacje o tej pozycji

Numer CAS:

9001-08-5

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-579-2

MDL number:

MFCD00081455

Numer WE:

3.1.1.8 (BRENDA, IUBMB)

Specific activity:

≥900 units/mg protein

Informacje o cenach i dostępności nie są obecnie dostępne.

Pomoc techniczna

Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.

Pozwól nam pomóc

Właściwości

form

lyophilized powder

Quality Segment

300

specific activity

≥900 units/mg protein

mol wt

tetramer 440 kDa

composition

Protein, ≥10%

solubility

H₂O: soluble 10 mg/mL

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Opis

Application

Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has been explored. It has been reported that the level of BChE in human blood correlates to the degree of protection against potentially toxic nerve agents. Cholinesterases have also been investigated for their role in Alzheimer′s disease.

Biochem/physiol Actions

Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities.BChE can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). The enzyme is activated by Ca²⁺ and Mg²⁺. The activity is constant over the pH range of 6.0-8.0. It is inhibited by betaine, nicotine, organophosphates, and carbamate.

Physical form

Highly purified; contains buffer salts

Analysis Note

Protein determined by biuret

Other Notes

One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Porównaj podobne pozycje

Wyświetl pełne porównanie

Pokaż różnice

1 of 1

Ta pozycja	C4290	SRE0020	C7512
Sigma-Aldrich C1057 Butyrylcholinesterase from equine serum	Sigma-Aldrich C4290 Butyrylcholinesterase from equine serum	Sigma-Aldrich SRE0020 Butyrylcholinesterase from equine serum	Sigma-Aldrich C7512 Butyrylcholinesterase from equine serum
description lyophilized powder, ≥900 units/mg protein	description lyophilized powder, ≥500 units/mg protein	description Suitable for manufacturing of diagnostic kits and reagents, ≥900 units/mg protein	description lyophilized powder, ≥10 units/mg protein
specific activity ≥900 units/mg protein	specific activity ≥500 units/mg protein	specific activity ≥900 units/mg protein	specific activity ≥10 units/mg protein
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
application(s) diagnostic assay manufacturing	application(s) -	application(s) diagnostic assay manufacturing	application(s) -
solubility H₂O: soluble 10 mg/mL	solubility -	solubility soluble (Native Sample buffer)	solubility cold water: 60 U/mL
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt tetramer 440 kDa	mol wt -	mol wt -	mol wt tetramer 440 kDa