D0184

2′-Deoxyuridine 5′-triphosphate sodium salt solution

100 mM in H₂O

• Synonim(y): dUTP sodium salt
• Wzór empiryczny (zapis Hilla): C₉H₁₅N₂O₁₄P₃ · xNa⁺
• Numer CAS: 94736-09-1
• Masa cząsteczkowa: 468.14 (free acid basis)
• NACRES: NA.52
• PubChem Substance ID: 24893218
• UNSPSC Code: 41106305
• MDL number: MFCD01630759
• Assay: ≥99%
• Form: liquid
• Storage temp.: −20°C

Właściwości

• assay: ≥99%
• Quality Segment: 200
• form: liquid
• concentration: 100 mM in H₂O
• technique(s): whole genome amplification: suitable
• color: colorless
• foreign activity: DNase, RNase, none detected
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=CC(=O)NC2=O
• InChI: 1S/C9H15N2O14P3.Na.H/c12-5-3-8(11-2-1-7(13)10-9(11)14)23-6(5)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,12H,3-4H2,(H,18,19)(H,20,21)(H,10,13,14)(H2,15,16,17);;
• InChI key: FVFWWPFKJNTHIQ-UHFFFAOYSA-N

Opis

General description

2′-Deoxyuridine 5′-triphosphate (dUTP) is incorporated into DNA during DNA synthesis. It is one of the main intermediate during pyrimidine nucleotide biosynthesis. It is one of the sources of uracil in DNA. Deoxyuridine 5′-triphosphate nucleotidohydrolase suppresses the incorporation of dUTP into DNA. It converts dUTP to 2′-deoxyuridine 5′-monophosphate (dUMP).

Application

2′-Deoxyuridine 5′-triphosphate sodium salt solution has been used in the mouse genomic DNA amplification by polymerase chain reaction (PCR). It is also suitable for conjunction with uracil DNA glycosylase to prevent carryover contamination in PCR. The dUTP is substituted for dTTP in the PCR reaction.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable