Likely temperature needs to be optimized. It is suggested to try a range of temperatures (37, 40, 43) and to adjust stringency conditions if the background is too high.
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H7033
PerfectHyb™ Plus Hybridization Buffer
for Northern and Southern blotting, solution
Synonim(y):
hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|
Informacje o tej pozycji
NACRES:
NA.25
UNSPSC Code:
12161700
Informacje o cenach i dostępności nie są obecnie dostępne.
Pomoc techniczna
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Pozwól nam pomócGeneral description
Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.
Application
Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.
Features and Benefits
- Fast and easy-to-use
- Up to 20x more sensitive than conventional buffers
- Doesn′t require blocking steps
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent, colorimetric , and DIG detection assays
- Compatible with radioactive and non-radioactive probes
- Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes
Other Notes
A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.
Legal Information
PerfectHyb is a trademark of Sigma-Aldrich Co. LLC
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Ta pozycja | |||
|---|---|---|---|
| description for Northern and Southern blotting, solution | description - | description for Northern and Southern blotting, liquid (2x concentrate) | description - |
| Quality Level 200 | Quality Level 200 | Quality Level 100, 200 | Quality Level 100 |
| form solution | form liquid | form liquid (2x concentrate) | form - |
| storage temp. room temp | storage temp. −20°C | storage temp. -10 to -25°C | storage temp. - |
Klasa składowania
12 - Non Combustible Liquids
Co się dzieje?
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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When using H7033 with a 122-base long oligo as a probe for hybridization, should the DNA hybridization temperature or the high end of the oligonucleotide temperature be used?
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