This product may be prepared in 50 mM TES buffer, pH 7.0, with 1 mM MgCl2, at 1 mg/mL. It is also soluble in water or TE buffer. Please see the link below to review the product datasheet for additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/382/514/m9901pis-ms.pdf
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M9901
Mutanolysin from Streptomyces globisporus ATCC 21553
lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified
Synonim(y):
Mutanolysin
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 311,00 zł |
5000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 757,00 zł |
10000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1080,00 zł |
50000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3050,00 zł |
Informacje o tej pozycji
Specific activity:
≥4000 units/mg protein (biuret)
Biological source:
bacterial (Streptomyces globisporus)
311,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
bacterial (Streptomyces globisporus)
Quality Segment
form
lyophilized powder
specific activity
≥4000 units/mg protein (biuret)
mol wt
23 kDa
input
bacteria and yeast sample(s)
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
General description
Application
Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Biochem/physiol Actions
Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.
Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.
Physical form
Lyophilized powder containing Ficoll® and sodium succinate buffer salts
Other Notes
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Legal Information
Ficoll is a registered trademark of Cytiva
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Ta pozycja | |||
|---|---|---|---|
| description lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified | description Suitable for manufacturing of diagnostic kits and reagents, ≥4000 units/mg protein (biuret) | description free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret) | description 0.2 μm filtered, lyophilized powder, ≥4000 units/mg protein (biuret) |
| specific activity ≥4000 units/mg protein (biuret) | specific activity ≥4000 units/mg protein (biuret) | specific activity ≥4000 units/mg protein (biuret) | specific activity ≥4000 units/mg protein (biuret) |
| biological source bacterial (Streptomyces globisporus) | biological source bacterial (Streptomyces globisporus) | biological source Streptomyces sp. (Streptomyces globisporus ATCC 21553) | biological source - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) life science and biopharma | application(s) - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| suitability suitable for cell lysis | suitability suitable for cell lysis | suitability suitable for cell lysis | suitability - |
| mol wt 23 kDa | mol wt 23 kDa | mol wt ~18.5 kDa, 23 kDa | mol wt 23 kDa |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Ostrzeżenia
Hazard Classifications
Resp. Sens. 1
Klasa składowania
13 - Non Combustible Solids
Co się dzieje?
WGK 1
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, type N95 (US)
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Hi, Could you advise the best way to resuspend mutanolysin M9901. Will it be soluble in water?
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