N7653

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

• Synonim(y): 4-Nitrophenyl phosphate disodium salt solution, pNPP
• Wzór liniowy: O₂NC₆H₄OP(O)(ONa)₂
• Numer CAS: 4264-83-9
• Masa cząsteczkowa: 263.05
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897835
• NACRES: NA.83
• MDL number: MFCD00007319

Właściwości

• Nazwa produktu: p-Nitrophenyl Phosphate Liquid Substrate System, liquid
• form: liquid
• Quality Segment: 200
• technique(s): protein detection: suitable
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
• InChI key: VIYFPAMJCJLZKD-UHFFFAOYSA-L

Opis

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.

Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Physical form

Ready-to-use.

Disclaimer

Stable at least one year when stored below 0 °C.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS05
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H290,H315,H318,H361fd,H373
• Ostrzeżenia: P202 - P234 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
• Narządy docelowe: Kidney,Liver,Blood
• Klasa składowania: 12 - Non Combustible Liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable