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P3375
Papain from papaya latex
crude powder, 1.5-10 units/mg solid
Synonim(y):
Papainase
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
25 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 435,00 zł |
100 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 921,00 zł |
1 kg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3770,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
Numer WE:
Specific activity:
1.5-10 units/mg solid
435,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócform
crude powder
Quality Segment
specific activity
1.5-10 units/mg solid
mol wt
23.4 kDa
storage temp.
2-8°C
Application
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
Papain from papaya latex has been used:
- in the enzyme cocktail to evaluate the efficacy of B complex vitamers liberation from nutritional yeast powder
- in the enzyme mixture for the digestion of bovine milk samples for the analysis of three vitamin B1 molecules
- as a biocatalyst for the hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P3375, papain crude powder, has been used to digest fresh cartilage of dogsfish in order to isolate chondroitin sulfate.
Biochem/physiol Actions
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Analysis Note
Not standardized with lactose or other adulterants.
Other Notes
One unit will hydrolyze one micromole of N-alpha-benzoyl-l-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.
View more information on papain at www.sigma-aldrich.com/enzymeexplorer.
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Ta pozycja | |||
|---|---|---|---|
| description crude powder, 1.5-10 units/mg solid | description solution, light brown, ≥10 U/mg protein (~25 mg/ml) | description powder, ≥7,500 BAEE units/mg solid | description powder, ≥0.1 unit/mg solid |
| specific activity 1.5-10 units/mg solid | specific activity ≥10 U/mg protein (~25 mg/ml) | specific activity ≥7,500 BAEE units/mg solid | specific activity ≥0.1 unit/mg solid |
| form crude powder | form solution | form powder | form powder |
| mol wt 23.4 kDa | mol wt - | mol wt 23.8 kDa | mol wt 23.8 kDa |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 100 | Quality Level 400 | Quality Level 200 |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Ostrzeżenia
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Narządy docelowe
Respiratory system
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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