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SEQXE

SeqPlex DNA Amplification Kit

For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

Synonim(y):

SeqPlex Enhanced DNA Amplification Kit, WGA kit, whole genome amplification kit

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10 REACTIONS

1680,00 zł

50 REACTIONS

7750,00 zł

500 REACTIONS

51 350,00 zł

1680,00 zł

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Informacje o tej pozycji

NACRES:
NA.55
UNSPSC Code:
41121800

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technique(s)

whole genome amplification: suitable

Quality Level

200

dilution

(WGA)

input

purified DNA

shipped in

wet ice

storage temp.

−20°C

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Ta pozycja
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SeqPlex DNA Amplification Kit For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

Sigma-Aldrich

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WGA4

GenomePlex® Single Cell Whole Genome Amplification Kit

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

SeqPlex™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD™, or 454 sequencing workflows.

Application

SeqPlex™ DNA Amplification Kit has been used for whole genome amplification.[1][2][3]

Features and Benefits

  • Random priming technology amplifies fragmented DNA such as ChIP or FFPE
  • Facilitates sequencing from as little as 100 pg of ChIP DNA
  • Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
  • Compatible with Illumina®, SOLiD™, or 454 library prep for next generation sequencing

Other Notes

SEQXE-500RXN is manufactured on-demand. Contact technical services at [email protected] for more information.

Legal Information

Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
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Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki
  • W4502Water, Nuclease-Free Water, for Molecular BiologyKarta charakterystyki
  • Library Preparation BufferKarta charakterystyki

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used together

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Klasa składowania

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000505078
0000505078
0000482551
0000482551
0000482543

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Joshua A Udall et al.
Frontiers in plant science, 10, 1541-1541 (2019-12-13)
One of the extraordinary aspects of plant genome evolution is variation in chromosome number, particularly that among closely related species. This is exemplified by the cotton genus (Gossypium) and its relatives, where most species and genera have a base chromosome
Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Takuya Hayakawa et al.
Cytogenetic and genome research, 161(8-9), 437-444 (2021-11-25)
E/L Repli-seq is a powerful tool for detecting cell type-specific replication landscapes in mammalian cells, but its potential to monitor DNA replication under replication stress awaits better understanding. Here, we used E/L Repli-seq to examine the temporal order of DNA
Syuzo Kaneko et al.
Cancers, 13(9) (2021-05-01)
Although chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) using formalin-fixed paraffin-embedded tissue (FFPE) has been reported, it remained elusive whether they retained accurate transcription factor binding. Here, we developed a method to identify the binding sites of the insulator transcription factor
Isolation and sequencing of active origins of DNA replication by nascent strand capture and release (NSCR).
Kunnev D, et al.
Journal of biological methods, 2(4) (2015)
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Protokoły

Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

Protokół zestawu do amplifikacji DNA SeqPlex

Zestaw SeqPlex DNA Amplification Kit umożliwia NGS z małych lub zdegradowanych ilości DNA w celu amplifikacji całego genomu.

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