Wszystkie zdjęcia(2)

T6146

Sigma-Aldrich

Trypan Blue

powder, BioReagent, suitable for cell culture

Synonim(y):
Direct blue 14
Wzór liniowy:
C34H24N6O14S4Na4
Numer CAS:
Masa cząsteczkowa:
960.81
Numer w indeksie barw:
23850
Beilstein:
4360496
Numer EC:
Numer MDL:
Identyfikator substancji w PubChem:
NACRES:
NA.75

Poziom jakości

200

linia produktu

BioReagent

forma

powder

skład

Dye content, ~40%

opakowanie

glass bottle of 100 g
glass bottle of 25 g
glass bottle of 5 g

warunki przechowywania

dry at room temperature

technique(s)

cell culture | mammalian: suitable
tissue processing: suitable

mp

>300 °C (lit.)

rozpuszczalność

H2O: 10 mg/mL

application(s)

cell analysis

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

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Opis ogólny

The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.

Zastosowanie

Trypan blue has been used:
  • in cell viability assay
  • to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
  • to stain plant tissue in order to to visualize cell death

Opakowanie

5, 25, 100 g in glass bottle

Działania biochem./fizjol.

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

piktogramy

Health hazard

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Carc. 1B

Kod klasy składowania

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Środki ochrony indywidualnej

Eyeshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

Certyfikat analizy

Świadectwo pochodzenia

Plant tissue trypan blue staining during phytopathogen infection.
Fernandez NB, et al.
Bio-protocol (2016)
Acanthamoeba: Biology and Pathogenesis, 85-85 (2009)
Trypan blue exclusion test of cell viability
Strober W
Current Protocols in Immunology, 21(1), A-3B (1997)
Knockdown of PSMB7 induces autophagy in cardiomyocyte cultures: possible role in endoplasmic reticulum stress
Kyrychenko VO. et al.
Pathobiology, 81(1), 8-14 (2014)
A histological analysis of lens capsules stained with trypan blue for capsulorrhexis in phacoemulsification cataract surgery
Singh AJ, et al.
Eye (London, England), 17(5), 567-567 (2003)

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