T9549

Thrombin from bovine plasma

powder, suitable for cell culture, ≥1,500 NIH units/mg protein (E1%/280 = 19.5)

• Synonim(y): Factor IIa
• Numer CAS: 9002-04-4
• UNSPSC Code: 12352204
• EC Number: 232-648-7
• MDL number: MFCD00166053
• Numer WE: 3.4.21.5 (BRENDA, IUBMB)
• Specific activity: ≥1,500 NIH units/mg protein (E1%/280 = 19.5)

Właściwości

• form: powder
• specific activity: ≥1,500 NIH units/mg protein (E1%/280 = 19.5)
• mol wt: heavy chain ~31 kDa, light chain ~6 kDa
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P00735
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: cow ... F2(280685)

Opis

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess an expression and purification system for the biosynthesis of adenosine receptor peptides for biophysical and structural characterization.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Preparation Note

When reconstituted with 1 mL water, vial contains stated activity in 0.15 M sodium chloride and 0.05 M sodium citrate, pH 6.5.

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

Activity is expressed in NIH units, and is measured by direct comparison to an NIH thrombin reference standard.

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H315,H319,H334,H335
• Ostrzeżenia: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Narządy docelowe: Respiratory system
• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable