Health assessment of gasoline and fuel oxygenate vapors: micronucleus and sister chromatid exchange evaluations.

Regulatory toxicology and pharmacology : RTP (2014-05-24)
Ceinwen A Schreiner, Gary M Hoffman, Ramadevi Gudi, Charles R Clark

Micronucleus and sister chromatid exchange (SCE) tests were performed for vapor condensate of baseline gasoline (BGVC), or gasoline with oxygenates, methyl tert-butyl ether (G/MTBE), ethyl tert butyl ether (G/ETBE), t-amyl methyl ether (G/TAME), diisopropyl ether (G/DIPE), t-butyl alcohol (TBA), or ethanol (G/EtOH). Sprague Dawley rats (the same 5/sex/group for both endpoints) were exposed to 0, 2000, 10,000, or 20,000mg/m(3) of each condensate, 6h/day, 5days/week over 4weeks. Positive controls (5/sex/test) were given cyclophosphamide IP, 24h prior to sacrifice at 5mg/kg (SCE test) and 40mg/kg (micronucleus test). Blood was collected from the abdominal aorta for the SCE test and femurs removed for the micronucleus test. Blood cell cultures were treated with 5μg/ml bromodeoxyuridine (BrdU) for SCE evaluation. No significant increases in micronucleated immature erythrocytes were observed for any test material. Statistically significant increases in SCE were observed in rats given BGVC alone or in female rats given G/MTBE. G/TAME induced increased SCE in both sexes at the highest dose only. Although DNA perturbation was observed for several samples, DNA damage was not expressed as increased micronuclei in bone marrow cells. Inclusion of oxygenates in gasoline did not increase the effects of gasoline alone or produce a cytogenetic hazard.

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Opis produktu

tert-Butyl methyl ether, ACS reagent, ≥99.0%
tert-Butyl methyl ether, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%
tert-Butyl Methyl Ether, Pharmaceutical Secondary Standard; Certified Reference Material
tert-Butyl methyl ether, suitable for HPLC, ≥99.8%
tert-Butyl methyl ether, reagent grade, ≥98%
tert-Butyl methyl ether, analytical standard
tert-Butyl methyl ether, anhydrous, 99.8%