Analiza HPLC mieszaniny testowej na kolumnie Ascentis^® Express RP-Amide

column

Ascentis Express RPA 15 cm x 4.6 mm, 5 μm particles (50773-U)

mobile phase

[A] 20 mM potassium phosphate, pH 7; [B] acetonitrile; (70:30, A:B)

flow rate

4.0 mL/min

pressure

4467 psi (308 bar)

column temp.

26 °C

detector

UV, 254 nm

injection

5 μL

sample

1. Uracil (0.016 mg/mL), 2. Benzamide (0.161 mg/mL), 3. Aniline (0.161 mg/mL), 4. Cinnamyl Alcohol (0.016 mg/mL), 5. Dimethyl Phthalate (0.323 mg/mL), (6. 2-Nitroaniline (0.081 mg/mL), 7. 4′-Bromoacetanilide (0.040 mg/mL), 8. 2,2′-Biphenol (0.161 mg/mL), 9. 4,4′-Biphenol (0.040 mg/mL), in 50/50 Water/Acetonitrile)