04-037

Anti-RalB Antibody, clone 25

clone 25, Upstate^®, from mouse

• Synonym(s): RAS-like protein B, v-ral simian leukemia viral oncogene homolog B, GTP binding protein, v-ral simian leukemia viral oncogene homolog B
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 25, monoclonal
• Application: WB
• Citations: 17

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 25, monoclonal
• species reactivity: mouse, rat, human
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_002881.2
• UniProt accession no.: P11234
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RALB(5899)

Description

General description

24 kDa

RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarizatied exocystossis in epithelaial cells (Cascone, 2008). RalB is required for metastatic formation and cell survival by driving exocyst assembly during polarized cell migration (Cascone, 2008). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes

Immunogen

Epitope: C-terminus

Synthetic peptide of the C-terminus of RalB

Application

Detect RalB using this Anti-RalB Antibody, clone 25 validated for use in WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

G-proteins

Biochem/physiol Actions

Other species have not been tested.

This antibody is specific for RalB and does not detect RalA.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Evaluated by western blot on HEK293 or NIH/3T3 cell lysates.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect RalB in HEK293, NIH/3T3, and rat liver cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable