The typical working concentration of DPP-IV Inhibitor is 10 µL per 1 mL of whole blood, resulting in a final inhibitor concentration of 50 µM. For 2.7 mL of blood, add 27 µL of inhibitor. For approximately 160 samples, the total volume required is 4.32 mL (27 µL per sample × 160 samples). This product has not been tested for suitability in ELISA. However, it has been used successfully by other researcher in this application. Please see the link below to review a publication that may be of interest:
https://pmc.ncbi.nlm.nih.gov/articles/PMC3138313/
DPP4-M
DPP IV Inhibitor
Synonym(s):
DPP IV Inhibitor, Dipeptidyl peptidase 4 Inhibitor, Gliptins
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Check Cart for Availability | $82.30 |
10 mL | Check Cart for Availability | $631.00 |
About This Item
UNSPSC Code:
41116012
NACRES:
NC.07
eCl@ss:
32160405
form
solution
Quality Segment
NCBI accession no.
UniProt accession no.
Gene Information
human ... DPP4(1803)
General description
Application
Research Category
All
All
Biochem/physiol Actions
Dipeptidyl peptidase IV (DPP IV) controls insulin-stimulating hormones, glucagon-like peptide (GLP-1), and glucose-dependent insulinotropic polypeptide (GIP). It′s a promising therapeutic target for type 2 diabetes (T2DM). In addition to its catalytic action, DPP-4 also serves as a binding protein and a ligand of extracellular factors. DPP-4 inhibition causes GLP-1 and GIP to have a prolonged activity, hence DPP-4 inhibitors help maintain glucose homeostasis. DPP IV inhibitors can improve glycemic control for a longer period when compared to early oral hypoglycemics.
Preparation Note
Upon arrival, store at 4°C. For long-term of more than 2 weeks, store at –20°C.
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This Item | |||
|---|---|---|---|
| description - | description recombinant, expressed in baculovirus infected Sf9 cells, pkg of ≥1.0 units/vial, ≥10 units/mg protein | description recombinant, expressed in Sf9 cells | description - |
| Gene Information human ... DPP4(1803) | Gene Information human ... DPP4(1803) | Gene Information human ... DPP4(1803) | Gene Information - |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form solution | form solution | form solid |
| NCBI accession no. | NCBI accession no. - | NCBI accession no. - | NCBI accession no. - |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. - |
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Sehr geehrte Damen und Herren, welche Menge Ihres DPP-4-Inhibitors empfehlen Sie für 2,7 ml Blut zur GLP-1-Bestimmung? Ist er für aktives GLP-1 mittels ELISA geeignet? Wir planen ca. 160 Proben.
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What is the stability of this compound at 4C? How many freeze thaws can it tolerate?
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This product is stable in 4 degree C for at least 2 weeks. Freeze-thaw cycles should be kept to a minimum. Up to three cycles have been performed without any detectable loss of activity. To further protect product integrity, aliquoting the material is recommended to avoid repeated freezing and thawing. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/991/950/dpp4-insert.pdfHelpful?
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Would our lab be able to store this product at -80 degrees celsius without compromising its composition?
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It is recommended to store this product at -20°C. The long term stability of this product at -80°C has not been determined. However, there are no issues anticipated by storing this material at ultra-low temperatures.
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What is the concentration of this product and what is the working concentration suggested? Thanks!
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This product as provided at a concentration of 4 mM. The working concentration recommendations are 10 μl/mL of whole blood or 20 μl/mL of buffer, plasma, serum, or tissue culture medium. These are general usage recommendations, the optimal concentration is application- and/or system-dependent. The final working concentration should be empirically determined by the end user.
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