SCT032

BioTracker 515 Green Zn2+ DA Dye

Live cell imaging dye for zinc (Zn2+) for cell signaling, protein self-assembly and cellular metabolism applications.

• Synonym(s): Live cell imaging probe
• UNSPSC Code: 12352207
• NACRES: NA.47
• eCl@ss: 32161000

Properties

• technique(s): cell based assay: suitable, live cell imaging : suitable
• detection method: fluorometric
• shipped in: ambient
• storage temp.: -10 to -25°C

Description

General description

Zinc (Zn2+) is a trace nutrient critical for physiological function. Although most biological zinc ions are tightly associated with proteins, pools of loosely bound or mobile forms serve regulatory or signaling functions, including nucleation of protein self-assembly, triggering of signaling pathways, and modification of cellular metabolism.

The BioTracker 515 Green Zn2+ Dye is a fluorescent imaging probe for the detection of Zn2+ in living cells. The BioTracker 515 Green Zn2+ Dye has high solubility, so that it is not permeable through the cell membrane. BioTracker 515 Green Zn2+ Dye DA has an additional acetyl group which make the reagent go inside the cell much easier, and the acetyl group is removed by the intracellular esterase which make the reagent stay inside the cell for extended periods of time.

Concentration of the provided sample is 5 mmol/L in DMSO. Dilute 1000 times with neutral buffer before use.

Spectral Properties
Absorbance: 492nm
Emission: 515nm

Application

Live cell fluorescent imaging

Research Category
Cell Imaging

Research Sub Category
Live Cell Dye

Physical form

Lyophilized

Preparation Note

Store BioTracker 515 Green Zn2+ DA Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): 849.6 °F
• Flash Point (°C): 454.2 °C