A1908

Asialofetuin from fetal calf serum

Type II (Sigma designation)

• UNSPSC Code: 12352202
• NACRES: NA.61
• MDL number: MFCD00130561
• Form: powder
• Biological source: bovine

Properties

• biological source: bovine
• Quality Segment: 200
• type: Type II (Sigma designation)
• form: powder
• technique(s): activity assay: suitable, inhibition assay: suitable
• impurities: salt, essentially free, ≤1.0% N-acetylneuraminic acid
• solubility: H₂O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow
• storage temp.: 2-8°C

Description

Application

Asialofetuin from fetal calf serum is suitable for use:

• in the assay of the lactose binding activity of the galectin-7 by immunodetection of in situ binding activity to asialofetuin immobilized on nitrocellulose[1]
• in the inhibition of zona pellucida hardening of mouse oocytes[2]
• as an acceptor in sialyltransferase enzyme activity assay[3]

Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.

Biochem/physiol Actions

Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain.[4] It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.[5]

Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.

Preparation Note

Enzymatic cleavage using neuraminidase

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)