B7405

Anti-Digoxin−Biotin antibody, Mouse monoclonal

clone DI-22, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Digoxin, Monoclonal Anti-Digoxin−Biotin antibody produced in mouse
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: biotin conjugate
• Clone: DI-22, monoclonal
• Application: ELISA (i), WB
• Citations: 10

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: biotin conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: DI-22, monoclonal
• form: buffered aqueous solution
• technique(s): indirect ELISA: 1:10,000 using digoxin-labeled biomolecule, western blot: 1:500 using using digoxin-labeled biomolecule
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin-Biotin antibody produced in mouse has been used in:

• in situ hybridization
• immunohistochemistry
• immunolocalization

Monoclonal Anti-Digoxin-Biotin antibody produced in mouse may be used for ELISA at a dilution of 1:10,000 and for dot blots at a dilution of 1:500. It was also used to stain apoptotic cells in cervical carcinoma sections by TUNEL technique.

Biochem/physiol Actions

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.

The conjugate may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable