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D0690

DNA Gyrase from Escherichia coli

aqueous glycerol solution

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100 units
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$449.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
5.99.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00211877
Biological source:
Escherichia coli
Concentration:
≥2 unit/μL

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biological source

Escherichia coli

Quality Segment

form

aqueous glycerol solution

mol wt

~374 kDa

concentration

≥2 unit/μL

technique(s)

cell based assay: suitable

UniProt accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Application

DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.

Biochem/physiol Actions

DNA gyrase is supplied as an A2B2 holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA. DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.
Can be used to supercoil plasmids.

Other Notes

One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 °C.
Solution in 50% Glycerol containing Tris buffer, DTT and EDTA.

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DNA Gyrase from Escherichia coli aqueous glycerol solution

Sigma-Aldrich

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Sigma-Aldrich

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Sigma-Aldrich

A7437

Acetate Kinase from Escherichia coli

Nitroreductase from Escherichia coli ≥90% (SDS-PAGE), recombinant, expressed in E. coli

Sigma-Aldrich

N9284

Nitroreductase from Escherichia coli

description

aqueous glycerol solution

description

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

description

lyophilized powder, ≥150 units/mg protein (biuret)

description

≥90% (SDS-PAGE), recombinant, expressed in E. coli

Gene Information

Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Gene Information

Escherichia coli K12 ... trxA(948289)

Gene Information

Escherichia coli K12 ... ackA(946775)

Gene Information

Escherichia coli K12 ... nfsB(945483)

biological source

Escherichia coli

biological source

Escherichia coli

biological source

-

biological source

-

technique(s)

cell based assay: suitable

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

-

form

aqueous glycerol solution

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

lyophilized powder

concentration

≥2 unit/μL

concentration

-

concentration

-

concentration

-

application(s)

cell analysis

application(s)

cell analysis

application(s)

-

application(s)

-

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000575022
    0000564294
    0000564296
    SLCH7585
    SLCF6602

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