The capture and detection antibodies are mouse monoclonal antibodies raised against native Ferritin. The immunogen is native purified Ferritin from human liver.
RAB0197
Human Ferritin ELISA Kit
for serum, plasma, cell culture supernatant and urine
Synonym(s):
FTH, Ferritin H subunit, Ferritin heavy chain
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery) | $567.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
41116158
eCl@ss:
32160702
Gene information:
human ... FTH1(2495)
Input:
sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type cell culture supernatant(s)
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
$567.00
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
species reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, capture ELISA: suitable
input
sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 600 pg/mL
standard curve range: 0.81-200 ng/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... FTH1(2495)
General description
The Human Ferritin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ferritin in serum, plasma, cell culture supernatants and urine.
Ferritin is a heteropolymer globular protein of 450 kDa and is localized to the nucleus, mitochondria and cytoplasm. Ferritin is an intracellular protein containing 24 subunits (heavy and light).
Ferritin is a heteropolymer globular protein of 450 kDa and is localized to the nucleus, mitochondria and cytoplasm. Ferritin is an intracellular protein containing 24 subunits (heavy and light).
Immunogen
Recombinant Human Ferritin
Application
Human Ferritin ELISA Kit has been used to estimate the amount of ferritin in cellular suspension.
Human ferritin ELISA kit. has been used to measure the ferritin secreted in supernatants from THP-1 (tamm-horsfall protein 1) cells that were sequentially treated with lipopolysaccharides (LPS) and LLOMe (a lysosome disruption agent).
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Ferritin/ ferritin heavy chain 1 (FTH1) is a cytoplasmic protein. It acts as a cargo for transport to autophagosomes for degradation. FTH1 maintains the cellular redox status by storing the intracellular iron in a non-toxic and readily available form. It acts as a key antioxidant molecule in various physiologic and pathologic conditions, such as apoptosis, inflammation, vascular protection and neurodegeneration. FTH1 alone or along with ferritin light chain is implicated to detect cancer cells, stem cells, fibroblasts and dendritic cells.
Ferritin mediates iron metabolism and plays a key role in redox homeostasis. Ferritin possesses ferroxidase activity and maintains iron in its non-toxic form. Ferritin acts as an iron-storage protein and stores excessive intracellular iron that is not utilized for the production of heme. Also, iron is released from ferritin upon degradation.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
1 of 1
This Item | |||
|---|---|---|---|
| description for serum, plasma, cell culture supernatant and urine | description - | description whole antiserum, liquid | description ~1.0 mg/mL, clone FTH24, purified immunoglobulin |
| Gene Information human ... FTH1(2495) | Gene Information human ... FTH1(2495) | Gene Information human ... FTH1(2495) | Gene Information human ... FTH1(2495) |
| species reactivity human | species reactivity human, rat | species reactivity human | species reactivity human |
| assay range inter-assay cv: <12% | assay range - | assay range - | assay range - |
| input sample type urine | input - | input - | input - |
| technique(s) capture ELISA: suitable | technique(s) western blot: 1:500 - 1:2000 | technique(s) Ouchterlony double diffusion: 1:16 | technique(s) flow cytometry: 10-20 μg/test using HeLa cells, immunoblotting: 0.5-1.0 μg/mL using total cell extracts of HEK-293T cells overexpressing FTH1 (expected: ~26kDa)., indirect immunofluorescence: 5-10 μg/mL using HeLa cells |
| detection method colorimetric | detection method - | detection method - | detection method - |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
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I was just looking at your human ferritin ELISA kit (RAB0197-1KT) and I was just wondering if it was able to detect only the heavy or light ferritin subunits or if it only detects ferritin as a whole protein?
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