S0664

Monoclonal Anti-Syntaxin antibody produced in mouse

clone HPC-1, ascites fluid

• Synonym(s): Anti-HPC-1, Anti-P35-1, Anti-STX1, Anti-SYN1A
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164742
• Conjugate: unconjugated
• Clone: HPC-1, monoclonal
• Application: ARR, ICC, WB
• Citations: 133

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: HPC-1, monoclonal
• mol wt: antigen 35 kDa
• contains: 15 mM sodium azide
• species reactivity: rat, bovine, rabbit
• technique(s): immunocytochemistry: suitable using monolayer cultures of neonatal retina cells, microarray: suitable, western blot: 1:2,000 using crude preparation of synaptic vesicles from rat cerebral cortex
• isotype: IgG1
• UniProt accession no.: P32850
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Stx1a(116470)

Description

General description

Monoclonal Anti-Syntaxin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Syntaxin (also cited as HPC-1 antigen), a 35 kDa molecule with carboxyl-terminal membrane anchor is a synaptic protein. Syntaxin 1 is a membrane protein. Neurons express syntaxin 1A.

Syntaxin is a 35kD synaptic protein capable of interacting with the synaptic vesicle protein synaptotagmin. It facilitates the docking and fusion of synaptic vesicles with the plasma membrane. Monoclonal anti-syntaxin antibody can be used to screen λ gt 11 library of rat hippocampus to isolate syntaxin cDNA clones. It can also be used in microarray and immunocytochemistry. Mouse anti-syntaxin antibody reacts specifically with membrane protein syntaxin (35 kD). The product has also shown cross reactivity for rabbit, rat and bovine but not for guinea pig.

Immunogen

synaptosomal plasma-membrane fraction from adult rat hippocampus.

Application

Monoclonal Anti-Syntaxin antibody produced in mouse has been used in:

• immunoblotting
• immunohistochemistry
• immunoprecipitation from hippocampal lysate
• immunohistochemical analysis

Monoclonal anti-syntaxin antibody can be used in immunoprecipitation and immunohistochemistry. It can also be used in western blotting. Further, this product can also be used to label monolayer cultures of neonatal retinal cells and frozen paraformaldehyde fixed tissue sections.

Biochem/physiol Actions

Syntaxin 1 (STX1A) may control exocytosis in synaptic vesicles. In retina, STX1A may help in the structural formation of the inner nuclear layer (INL) and outer plexiform layer (OPL). Deletion of STX1A in mice affects the functions of hypothalamic-pituitary-adrenal axis. It may participate in spinal nociceptive plasticity promoted by peripheral nerve injury. It has been implicated in docking at synaptic vesicles of presynaptic neurotransmitter release sites.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable