- Highly specific targeting of human leukocytes using gold nanoparticle-based biologically active conjugates.
Highly specific targeting of human leukocytes using gold nanoparticle-based biologically active conjugates.
Journal of biomedical nanotechnology (2014-05-09)
Bernhard F Gibbs, Inna M Yasinska, Luigi Calzolai, Douglas Gilliland, Vadim V Sumbayev
PMID24804546
ABSTRACT
In this study we demonstrate for the first time a new approach for highly specific targeting of viable human leukocytes by functionalised gold nanoconjugates made on the basis of citrate-stabilised gold nanoparticles. This immobilisation involves highly specific recognition of the cells of interest by plasma membrane-associated receptor ligands or functional antibodies attached to gold nanoconjugates. Using this technology we managed to deliver pharmacological inhibitors into the target cells. Also we immobilized fully functional human leukocytes utilising both cell lines and rare primary human cells (basophils). This approach demonstrates possibilities of using nanoconjugates for diagnostics, drug delivery and immune therapy.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Gold coated glass cover slip, 99.999% (Au), diam. × thickness 15 mm × 130-170 μm, layer thickness 100 Å
Sigma-Aldrich
Gold coated glass cover slip, L × W × thickness 22 mm × 22 mm × 130-170 μm, square, layer thickness 100 Å, 99.999% (Au)
Gold, rod, 25mm, diameter 5.0mm, as drawn, 99.95%
Gold, tube, 50mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%
Gold, rod, 10mm, diameter 5.0mm, as drawn, 99.95%
Gold, rod, 25mm, diameter 2.0mm, as drawn, 99.95%
Gold, rod, 50mm, diameter 2.0mm, as drawn, 99.95%
Gold, tube, 50mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%
Gold, insulated wire, 0.5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%
Gold, tube, 200mm, outside diameter 1.0mm, inside diameter 0.7mm, wall thickness 0.15mm, as drawn, 99.95%
Gold, rod, 25mm, diameter 1.6mm, as drawn, 99.95%
Gold, sphere, 20pcs, diameter 0.5mm, 99.99%
Gold, tube, 100mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%
Gold, insulated wire, 2m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%
Gold, insulated wire, 5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
Gold, insulated wire, 5m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%
Gold, microfoil, disks, 10mm, thinness 1.0μm, specific density 1932μg/cm2, permanent mylar 3.5μm support, 99.99+%
Gold, tube, 50mm, outside diameter 0.3mm, inside diameter 0.2mm, wall thickness 0.05mm, as drawn, 99.95%
Gold, tube, 100mm, outside diameter 1.0mm, inside diameter 0.7mm, wall thickness 0.15mm, as drawn, 99.95%
Gold, rod, 10mm, diameter 3.0mm, as drawn, 99.95%
Gold, microleaf disks, 10mm, thinness 0.25μm, specific density 483μg/cm2, removable support, 99.99+%
Gold, insulated wire, 5m, conductor diameter 0.05mm, insulation thickness 0.009mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Gold, insulated wire, 1m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%
Gold, insulated wire, 0.2m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Gold, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
Gold, insulated wire, 5m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%
Gold, tube, 50mm, outside diameter 1.0mm, inside diameter 0.7mm, wall thickness 0.15mm, as drawn, 99.95%
Gold, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%