- Determination of the Juglone Content of Juglans regia Leaves by GC/MS.
Determination of the Juglone Content of Juglans regia Leaves by GC/MS.
Natural product communications (2015-09-29)
Irena Matławska, Wiesława Bylka, Ewa Widy-Tyszkiewicz, Beata Stanisz
PMID26411020
ABSTRACT
The constituents of walnut (Juglans regia L.) leaves are represented by tannins, phenolics, and naphthoquinones, the characteristic compound being juglone. The content of juglone in the methanolic extract of the leaves determined by the GC/MS method was 9.9 ± 0.2 mg/100 g; small amounts (1.3 ± 0.02 mg/100 g) were recorded in the infusion, whereas in the decoction it was not detected. As some studies indicate toxicity of juglone, only decoctions should be recommended for therapeutic use.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Methanol solution, suitable for NMR (reference standard), 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%