Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry.

Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry.

Journal of the American Oil Chemists' Society (2015-08-08)

Firas Jumaah, Margareta Sandahl, Charlotta Turner

ABSTRACT

A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO

MATERIALS

Product Number

Brand

Product Description

09-0800

Sigma-Aldrich

Ethanol, 94.8-95.8%

28-2280

Sigma-Aldrich

Sodium chloride, SAJ first grade, ≥99.0%

09-0790

Sigma-Aldrich

Ethanol, JIS special grade, 94.8-95.8%

28-2320

Sigma-Aldrich

Sodium chloride solution, 0.1 M

05-3390

Sigma-Aldrich

Chloroform, SAJ super special grade, ≥99.0%

05-3410

Sigma-Aldrich

Chloroform, SAJ first grade, ≥99.0%, contains 0.4-0.8% ethanol

05-3400

Sigma-Aldrich

Chloroform, JIS special grade, ≥99.0%

19-2450

Sigma-Aldrich

Methanol, JIS 300, ≥99.8%, suitable for residue analysis

05-3430

Sigma-Aldrich

Chloroform, JIS 300, ≥99.0%

28-2300

Sigma-Aldrich

Sodium chloride solution, 1 M

19-2420

Sigma-Aldrich

Methanol, SAJ first grade, ≥99.5%

28-2270

Sigma-Aldrich

Sodium chloride, JIS special grade, ≥99.5%

13-0360

Sigma-Aldrich

Heptane, JIS special grade, ≥99.0%

13-0370

Sigma-Aldrich

Heptane, SAJ first grade, ≥98.0%

717975

Sigma-Aldrich

Methanol, suitable for NMR (reference standard)

480150

Sigma-Aldrich

Chloroform, ACS reagent, ≥99.8%, contains amylenes as stabilizer

204439

Sigma-Aldrich

Sodium chloride, 99.999% trace metals basis

372978

Sigma-Aldrich

Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%

246654

Sigma-Aldrich

Heptane, anhydrous, 99%

288306

Sigma-Aldrich

Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer

378860

Sigma-Aldrich

Sodium chloride, random crystals, 99.9% trace metals basis

451940

Sigma-Aldrich

Sodium chloride-³⁵Cl, 99 atom % ³⁵Cl

19-2470

Sigma-Aldrich

Methanol, suitable for HPLC

05-3450

Sigma-Aldrich

Chloroform, suitable for HPLC

19-2400

Sigma-Aldrich

Methanol, SAJ special grade

S0817

Sigma-Aldrich

Sodium chloride solution, 0.85%

19-2410

Sigma-Aldrich

Methanol, JIS special grade, ≥99.8%

270512

Supelco

Heptane, HPLC grade, ≥99%

B1252

Sigma-Aldrich

Boron trifluoride-methanol solution, 14% in methanol

S6546

Sigma-Aldrich

Sodium chloride solution, 5 M