I9516

Sigma-Aldrich

2-Propanol

BioReagent, for molecular biology, ≥99.5%

Synonym(s):
IPA, Isopropanol, sec-Propyl alcohol, Isopropyl alcohol
Linear Formula:
(CH3)2CHOH
CAS Number:
Molecular Weight:
60.10
Beilstein/REAXYS Number:
635639
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

Quality Level

grade

for molecular biology

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C)
44 mmHg ( 25 °C)

product line

BioReagent

assay

≥99.5%

form

liquid

autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C

application(s)

DNA extraction: suitable
RNA extraction: suitable

impurities

≤0.2% water
water

color

colorless

refractive index

n20/D 1.377 (lit.)

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubility

water: soluble (completely)

density

0.785 g/mL at 25 °C (lit.)

suitability

suitable for precipitation of nucleic acids

storage temp.

room temp

SMILES string

CC(C)O

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

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General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

  • DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
  • Protein crystallization.
  • Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.

Application

2-Propanol has been used:
  • in DNA and RNA extraction
  • for determination of hydroxyproline levels(The Merck Index, 12th ed)
  • for checking cell viability by MTT assay
  • in microarray hybridization
Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.

Packaging

25, 500 mL in glass bottle

pictograms

FlameExclamation mark

signalword

Danger

hazcat

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

storage_class_code

3 - Flammable liquids

WGK Germany

WGK 1

Flash Point F

53.6 °F - closed cup

Flash Point C

12.0 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Zsuzsanna Rácz et al.
Nucleic acid therapeutics, 21(3), 215-224 (2011-07-14)
Short-interfering RNAs (siRNAs), key mediators of RNA interference comprise a promising therapeutic tool, although side effects such as interferon (IFN) response are still not perfectly understood. Further, delivery to target organs is a major challenge, possibly associated with side effects...
Christudas Morais et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 25(5), 1462-1474 (2009-12-29)
Renal cell carcinoma (RCC) is a highly metastatic and lethal disease with few efficacious treatments. Many studies have shown that the ubiquitous transcription factor nuclear factor kappa B (NF-kappaB) plays a key role in the development and progression of many...
Peter K Davidsen et al.
Journal of applied physiology (Bethesda, Md. : 1985), 110(2), 309-317 (2010-10-30)
MicroRNAs (miRNA), small noncoding RNA molecules, may regulate protein synthesis, while resistance exercise training (RT) is an efficient strategy for stimulating muscle protein synthesis in vivo. However, RT increases muscle mass, with a very wide range of effectiveness in humans....
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, E-E (1989)
M J Romão et al.
European journal of biochemistry, 215(3), 729-732 (1993-08-01)
The Desulfovibrio gigas aldehyde oxidoreductase contains molybdenum bound to a pterin cofactor and [2Fe-2S] centers. The enzyme was characterized by SDS/PAGE, gel-filtration and analytical ultracentrifugation experiments. It was crystallized at 4 degrees C, pH 7.2, using isopropanol and MgCl2 as...
Protocols
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
Read More
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
Read More
Related Content
Isopropanol is a polar organic solvent that is widely used in chemistry, biochemistry and molecular biology
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Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
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