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Merck

1.03840

Cellvento® 4CHO-X COMP

expansion medium

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3 L

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分類程式碼代碼:
41106213

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品質等級

500

描述

shipped with cooling packs

產品線

Cellvento®

形狀

dry powder

技術

cell culture | mammalian: suitable (suspension)

儲存溫度

2-8°C

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此商品
1.0379514390C1.03796
Cellvento® 4CHO-X COMP expansion medium

SAFC

1.03840

Cellvento® 4CHO-X COMP

Cellvento® 4CHO COMP chemically defined cell culture medium, ≤1.0 IU/mL endotoxin, pH 5.1-5.6(without supplement), pH 6.7-7.3(with supplement)

SAFC

1.03795

Cellvento® 4CHO COMP

Cellvento® 4CHO-C克隆培养基 Animal- component free, chemically defined

SAFC

14390C

Cellvento® 4CHO-C克隆培养基

Cellvento® 4Feed COMP chemically defined cell culture feed

SAFC

1.03796

Cellvento® 4Feed COMP

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

form

dry powder

form

dry powder

form

liquid

form

dry powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

product line

Cellvento®

product line

Cellvento®

product line

Cellvento®

product line

Cellvento®

description

shipped with cooling packs

description

shipped with cooling packs

description

-

description

shipped with cooling packs

Quality Level

500

Quality Level

500

Quality Level

-

Quality Level

500

一般說明

There are several advantages to using perfusion in your seed train, namely, flexibility in your production processes, increase in capacity and footprint reduction. Reducing the number of bioreactors in your seed train leads to a better utilization and eliminates traditional bottlenecks (compressed seed train). Additionally, using N-1 perfusion enables the inoculation of a production bioreactor with higher cell densities for high seed production process formats. Cellvento® 4CHO-X Expansion Medium has been specifically developed for N-1 perfusion for Chinese Hamster Ovary (CHO) cell lines. It is formulated with a high nutrient concentration to achieve low cell specific perfusion rates (CSPRs) at high cell densities. The medium is chemically defined, of non-animal origin, and contains no hydrolysates or components of unknown composition.

應用

Cellvento® 4CHO-X Expansion Medium has been specifically formulated to control the metabolic profile of the cells, both to minimize toxic byproducts and to ensure adequate nutrient levels for high cell densities. Preventing nutrient depletion can increase robustness and predictability of seed train operations. This medium supports high growth and viability, thus increasing cell biomass for cryopreservation or inoculation of another reactor. The medium addresses seed train intensification and shows a positive impact in IgG production, when combined in perfusion production with EX-CELL® Advanced HD Perfusion medium (24370C), and in Fed-batch production with e.g. Cellvento® 4CHO medium (103795) and Cellvento® 4Feed (103796). It is expected to work with other media platforms as well. Cellvento® 4CHO-X Expansion Medium can be used for all seed train expansion steps including N-1, reaching robust growth at low CSPRs. This product is intended for research or further manufacturing but not for human or therapeutic use.

準備報告

Formulated to contain 26.2 grams of powder per liter of medium.

法律資訊

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

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商品

Seed Train Intensification Using High Cell Density Cryopreservation

High cell density cryopreservation (HCDC) is a closed processing alternative to the traditional seed train expansion process. Learn more about the benefits of HCDC.

CHO Media and Feeds for Optimized Production of Therapeutic Proteins

Cellvento® and EX-CELL® CHO cell culture media and feeds, optimized for monoclonal antibody & recombinant protein production in fed-batch & perfusion

Establishing the First GMP Guideline for Cell Culture Media

Cell culture media are vital for bioprocessing therapeutics. This page describes the central role of our organization in authoring purpose-built GMP for cell culture media.

Optimizing CHO Cells for Higher Productivity

Cellvento 4CHO-X Expansion Medium enhances cell biomass for bioreactor inoculation in cell expansion stages.

相关内容

Production and Harvest Intensification

Biopharmaceutical developers need efficient, productive upstream processes that deliver high quality monoclonal antibodies (mAbs) and other types of recombinant proteins. Process intensification can future-proof their manufacturing strategies, reduce costs, and increase facility flexibility.

Seed Train Intensification

Biopharmaceutical developers need efficient, productive upstream processes that deliver high quality monoclonal antibodies (mAbs) and other types of recombinant proteins. Process intensification can future-proof their manufacturing strategies, reduce costs, and increase facility flexibility.

Cell Culture Media Development for mAbs

Learn more about cell culture media development for mAB development and manufacturing, and the products you need for this step.

生产和收获强化

生物制药开发商需要高效、高产的上游工艺来提供优质的单克隆抗体(mAb)和其他类型重组蛋白。工艺强化可确保生产策略适应未来发展需求、降低成本并提升设施灵活性。

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