The Scepter 3.0 sensors are single use only.
PHCC360KIT
Scepter 3.0手持式全自动细胞计数器
Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter 3.0 Cell Counter Sensors
Scepter 3.0 Handheld Automated Cell Counter
别名:
Cell counting instrument
跳至
製造商／商標名
Scepter
技術
cell counting: suitable
粒徑
6-36 μm
相容性
for use with cells sized 4.0–25.0 μm
運輸包裝
ambient
一般說明
Scepter 3.0手持式自动细胞计数器通过便捷的手持式设计，实现快速的细胞计数。Scepter 3.0仅供研究使用，并且已通过常用代表性细胞类型的测试。在制备目标细胞培养物的稀释液后，屏幕上的说明将引导您完成取样过程。在以Scepter 细胞计数器将样品加入Scepter 细胞计数器传感器之后，30秒钟以内Scepter 细胞计数器′的屏幕将显示：
Scepter 3.0细胞计数器兼容40uM 和 60uM Scepter 3.0 细胞计数器传感器。该套件（PHCC360KIT）包括一个免费的50-pack 60uM 传感器。
- 细胞浓度
- 细胞平均大小
- 细胞平均体积
- 大小或体积分布的直方图
Scepter 3.0细胞计数器兼容40uM 和 60uM Scepter 3.0 细胞计数器传感器。该套件（PHCC360KIT）包括一个免费的50-pack 60uM 传感器。
其他說明
- 1 台Scepter 3.0 自动手持式细胞计数器
- 1 个测试珠瓶
- 1 个充电站
- 50个传感器 （60μM）
Scepter 3.0细胞计数器不兼容上一代传感器；只兼容相关产品部分所示的3.0 细胞计数器传感器。
附屬品
产品编号
说明
价格
儲存類別代碼
10-13 - German Storage Class 10 to 13
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
商品
The Scepter™ 3.0 Cell Counter, when used in conjunction with a 40 μm aperture sensor, can accurately count and precisely size a wide range of cell types and particles. This application note demonstrates two examples of experiments using the instrument’s sensitive size-discriminating capacity for rapid qualitative assessment of individual cell populations.
Learn how Sterivex™ pressure-driven sterile filters can be used for large-volume sample processing in environmental research and testing.
Cell validation data using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.
Data for validated human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.
相关内容
A list of which mammalian and other key phenotypes have been validated for Scepter™ counting precision.
通过Scepter™计数精密度验证的哺乳动物和其他关键表型清单。
Scepter™ counting is 7 to 10 times faster than hemocytometry-and also faster than other automated counters. Learn more about how this amazing technology works
Streamline TEER data capture and analysis with user-friendly enhancements including an intuitive touchscreen interface, standing in-well probe, and automatic data logging.
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Can I confirm if the sensors are single-use only please?
1 answer-
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What is the maximum size of cell clumps that can pass through the Scepter 3.0? Also, what size screen is used to break up the cell clump aggregates in both Scepter 2.0 and 3.0?
1 answer-
The maximum size of cell clumps that can pass through the Scepter 3.0 is determined by the aperture limits, which are 40um and 60um for sensors 40 and 60, respectively. Unlike the Scepter 2.0, the Scepter 3.0 does not use a screen to break up cell clump aggregates; instead, it employs a set of pillars to achieve this function. In contrast, the Scepter 2.0 utilizes a 35um Nylon filter to break up cell clump aggregates.
Helpful?
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Is this appropriate for size 15-23 μm cell? Does the Kit include also the charger? How many 60/40 μm sensors are included Which sensors can be used with this instrument? Which sensors are appropriate for cells size 15-23 μm and what is their price?
1 answer-
Please see the answers to the following questions below:
1. Is this appropriate for size 15-23 μm cells?
Yes, the Scepter 3.0 is suitable. As listed on the product detail page in the DESCRIPTION section for use with cells sized 4.0–25.0 μm.2. Does the Kit include the charger?
This product does include 60μM sensors and a charging station.3. How many 60/40 μm sensors are included
As mentioned on the product detail page in the DESCRIPTION section under 'Components', it includes 50 Sensors.4. Which sensors can be used with this instrument?
This product is only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products on this page. Please see the link below to review additional information regarding the Scepter 3.0:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/scepter-cell-counter5. Which sensors are appropriate for cells size 15-23 μm and what is their price?
See question 1, this product is suitable for the cell size of 15-23 μm.Regarding pricing, Please navigate to the link https://www.sigmaaldrich.com/support/customer-support, and click on "Products Pricing & Availability" under the Products Section with all the required information so that a member of the Customer Service team can reach out to assist further. Thank you.
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Does it require Trypan blue or another dye? Does it distinguish between live and dead cells?
1 answer-
The Scepter is a particle counter, but it will not differentiate live from dead cells. The device can be used to gauge cell health on the basis of cell size. For example, in cell types where apoptotic cells are smaller in size compared to non-apoptotic cells. This shift in size can be quantified using the device option "Edit Histogram" to either gate around this population or exclude the population from the count.
Please see the following manual below for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/799/484/00110230w-rev1020-ms.pdfHelpful?
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