If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
产品名称
羟乙基哌嗪乙硫磺酸 溶液, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
無菌
sterile-filtered
品質等級
產品線
BioReagent
BioXtra
化驗
0.9—1.1 M
形狀
liquid
儲存條件
(Tightly closed. Dry)
dry at room temperature
濃度
1 M
技術
cell culture | mammalian: suitable
雜質
endotoxin, tested
顏色
colorless
pH值
7.0-7.6
有用的pH值範圍
6.8-8.2
7.5
密度
1.1 g/cm3
適合性
suitable for component for culture media
suitable for electrophoresis
應用
cell analysis
life science and biopharma
sample preparation
儲存溫度
2-8°C
SMILES 字串
OCCN1CCN(CCS(=O)(O)=O)CC1
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI 密鑰
JKMHFZQWWAIEOD-UHFFFAOYSA-N
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相关类别
1 of 4
此商品 | H3537 | H3537 | H4034 |
|---|---|---|---|
| assay 0.9—1.1 M | assay - | assay - | assay ≥99.5% (titration) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, competitive inhibition ELISA: suitable |
| concentration 1 M | concentration 1 M | concentration 1 M | concentration - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 400 |
| product line BioReagent, BioXtra | product line - | product line - | product line - |
| application(s) cell analysis | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) clinical research |
| assay 0.9—1.1 M | assay - | assay - | assay ≥99.5% (titration) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, competitive inhibition ELISA: suitable |
| concentration 1 M | concentration 1 M | concentration 1 M | concentration - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 400 |
| product line BioReagent, BioXtra | product line - | product line - | product line - |
| application(s) cell analysis | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) clinical research |
一般說明
此外,它可维持细胞生理pH,保护细胞免受呼吸引起的二氧化碳波动的影响,保证细胞稳态。这项能力更胜碳酸氢盐缓冲液(NaHCO3)。除了这些适合细胞培养的优异特性,HEPES也适于各种生物学和生物化学研究。也可用于免疫沉淀、细胞裂解、活细胞成像等技术,提供稳定受控的实验环境。功能多样、pH缓冲能力出色、与其他分子的互作力低,HEPES是多个研究领域中不可或缺的工具。
應用
特點和優勢
- 高纯度产品,适合生化和生物学研究
- 除菌过滤处理,并通过细胞培养测试
- 即用型溶液
- 适用pH范围:6.8 - 8.2,pKa 7.5(25 °C)
- 即用型溶液,无需配制,节省时间
準備報告
免責聲明
同類產品
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
实验方案
Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.
Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.
相关内容
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Product Information Sheet
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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Hello! Is it dissolved in H2O entirely? or does it have other surfactants?
1 answer-
This product is comprised of HEPES free acid in tissue culture grade water. No other additives or preservatives are present, including surfactants.
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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Bonjour, Est-ce que ce produit H0887 peut-être congelé à -80°C sans risque de le faire précipiter ?
1 answer-
It is best not to freeze the product as received. However, should the product be inadvertently frozen, it is best to thaw the product slowly. Do not place the frozen product in an incubator or water bath. Once thawed, inspect for any precipitates before use. A clear, colorless solution will be suitable for further use. Should this buffer be required to prepare the stock solution of another reagent that requires freezer storage, thaw slowly if possible.
Helpful?
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Active Filters
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