If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
P4417
Phosphate buffered saline
tablet, pH 7.2-7.6 (1 tablet/200 mL)
别名:
PBS
跳至
产品名称
Phosphate buffered saline, tablet
形狀
tablet
儲存條件
dry at room temperature
顏色
white
pH值
7.2-7.6 (1 tablet/200 mL)
7.4
溶解度
water: 1 tablet/200 mL
water: soluble
密度
1.02 g/cm3 at 20—25 °C
1.03 g/cm3 at 20—25 °C
應用
diagnostic assay manufacturing
histology
life science and biopharma
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此商品 | P5119 | P38135 | P3813 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form tablet | form liquid | form powder | form powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| density 1.02 g/cm3 at 20—25 °C, 1.03 g/cm3 at 20—25 °C | density - | density - | density 1.19 g/cm3 at 20—25 °C, 1.22 g/cm3 at 20—25 °C |
| storage condition dry at room temperature | storage condition - | storage condition - | storage condition dry at room temperature |
| solubility water: soluble | solubility - | solubility water: soluble | solubility water: soluble |
一般說明
除了细胞生物学领域外，PBS的多功能性还体现在各种应用中，如为实现最佳抗体-抗原相互作用而构建定制化ELISA缓冲液。此外，它可用作蛋白A/G色谱的高效缓冲液，有助于基于特定抗体结合纯化抗体。在血液学和生物化学操作过程中，PBS 在细胞和组织的有效清洗中起关键作用。总之，PBS是生物学和生物化学研究中不可缺少的工具，具有等渗性、缓冲能力、简单和稳定的特性。其在细胞生物学、试剂制备、蛋白质纯化和各种生物分析中的多样化应用体现了其在增进科学知识和理解中的重要性。
應用
特點和優勢
- 适用于各种研究应用的优质产品
- 可用作生物学和生物化学应用的缓冲液成分
- 易溶于水
準備報告
相關產品
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
商品
Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.
实验方案
细胞染色可分为四个步骤：细胞制备、固定、抗体孵育和评估。
ELISA实验方案提供了进行间接ELISA和捕获ELISA的详细说明。了解进行夹心式ELISA和间接ELISA实验的推荐产品和技术信息。
本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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After reconstitution of the tab, at which condition should I autoclave the PBS?
1 answer-
The standard parameters of 121°C for 15 minutes at 15 lbs of pressure would be sufficient to sterilize a Phosphate Buffered Saline (PBS) solution.
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How much should one tablet weigh for it to yield 200ml 1xPBS? Asking just to make sure we won't use chipped tablets.
1 answer-
Each tablet of this product should weigh between 1814.5 and 2005.5 milligrams.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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If I want to make the same buffer molarity as the product P4474-1L, how many tablets should I dissolve in 1L of DI water?
1 answer-
P4417 and P4474 have different components and initial concentrations. One P4417 tablet dissolved in 200 mL of deionized water yields a 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride at pH 7.4, 25 °C. P4474 contains 1.058 mM potassium phosphate monobasic, 154 mM sodium chloride, and 2.97 mM sodium phosphate dibasic dihydrate. Due to these differences, it is not feasible to directly match the molarity of P4474 by dissolving P4417 tablets.
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Going by instructions, 1 tab in 200 mL water gives a 1x PBS sol'n. The recipe I use for 1x PBS has the same salt concs. Sorry if this is a silly question, but can I make up a 3x PBS solution simply by dissolving 3 tablets in 200 mL of water?
1 answer-
These tablets have not been tested for solubility or dissolution time at a 3X concentration. The end user would have to determine solubility experimentally. Note that 10X prepared solutions are available and may easily be diluted to the desired concentration. Please see the link below to review several of these product options:
https://www.sigmaaldrich.com/search/pbs-10x?focus=products&page=1&perpage=30&sort=relevance&term=PBS%2010X&type=productHelpful?
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Hi! If with this product I want to do a 10x solution, do i dilute it in 2000 mL or first in 200mL and then do the dilution? Thanks!
1 answer-
Dissolve 1 tablet in 200 ml of water to obtain: 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4 At 25 °C. These instructions are for a 1 X PBS preparation and this product is only intended for this specific concentration. If a 10X concentrate is needed, there are options in the catalog, such as the following products : 79383, P7059, 6506-OP, and 6505-OP.
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Is it possible to obtain the salt components for product 6501-200TAB? Additionally, can you provide the composition details for other PBS tablets or powder products?
1 answer-
The compositions are as follows:
1. 6501-200TAB: This PBS contains 137mM NaCl, 2.7 mM KCl, and 10mM Na phosphate.
2. P3813 - powder - Packed in Pouch or PAK: Each pouch contains 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride, totaling 9.55 g per packet. This is a standard PBS formulation.
3. P4417- Tablet: When dissolved in 200 mL of deionized water, one tablet yields 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride, at pH 7.4 and 25 °C. This product contains the same formulation as P3813 on a per liter basis (excluding tableting fillers): 8 g/L Sodium Chloride, 0.2 g/L Potassium Phosphate, monobasic, 1.15 g/L Sodium Phosphate, dibasic, and 0.2 g/L Potassium Chloride. This mixture should be diluted to one liter of water, resulting in a pH of 7.4, which can be adjusted using phosphoric acid or sodium hydroxide. The concentrations are 10 mM phosphate buffer, 2.7 mM potassium chloride, and 137 mM. These PBS tablets do not contain BSA.
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What is the osmolality of P4417 made to 1X PBS at pH 7.4?
1 answer-
This PBS once diluted in 200 mL distilled water will account for:
2.7 mM potassium chloride = 5.4 mosmoles / L
137 mM sodium chloride = 274 mosmoles / LThe exact osmolality is not determined.
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Is this PBS calcium-free?
1 answer-
The formulation of this product is considered proprietary. However, calcium is not included in the composition.
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