04-1643

Rabbit Anti-Mouse IgG Antibody, clone RM104

Name: Rabbit Anti-Mouse IgG Antibody, clone RM104
Brand: MM

clone RM104, from rabbit

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分類程式碼代碼:

12352203

eCl@ss:

32160702

NACRES:

NA.46

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生物源

rabbit

品質等級

100

抗體表格

purified antibody

抗體產品種類

secondary antibodies

無性繁殖

RM104, monoclonal

物種活性

mouse

技術

ELISA: suitable
western blot: suitable

同型

IgG

運輸包裝

wet ice

目標翻譯後修改

unmodified

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本產品	06-371	04-1648	SAB5600195
Sigma-Aldrich 04-1643 Rabbit Anti-Mouse IgG Antibody, clone RM104	Sigma-Aldrich 06-371 兔抗小鼠IgG抗体	Sigma-Aldrich 04-1648 Goat Anti-Rabbit IgG Fab Antibody, clone RMG01	Sigma-Aldrich SAB5600195 Anti-Mouse IgG antibody, Rabbit monoclonal
clone RM104, monoclonal	clone polyclonal	clone RMG01, monoclonal	clone RM104, monoclonal, recombinant monoclonal
biological source rabbit	biological source rabbit	biological source goat	biological source -
species reactivity mouse	species reactivity mouse	species reactivity rabbit	species reactivity mouse
antibody form purified antibody	antibody form purified immunoglobulin	antibody form purified antibody	antibody form purified immunoglobulin
technique(s) ELISA: suitable, western blot: suitable	technique(s) immunoprecipitation (IP): suitable	technique(s) ELISA: suitable, western blot: suitable	technique(s) ELISA: 0.005-0.2 μg/mL, immunocytochemistry: 0.5-2 μg/mL, immunohistochemistry: 0.5-2 μg/mL, immunoprecipitation (IP): suitable (assay dependent concentration), western blot: 0.1— 0.5 μg/mL
shipped in wet ice	shipped in dry ice	shipped in wet ice	shipped in wet ice

免疫原

Mouse IgG

應用

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

This rabbit monoclonal Rabbit Anti-Mouse IgG, clone RM104, Cat. No. 04-1643 is validated for use in ELISA and Western Blotting, for the detection of Mouse IgG.

Western Blotting Analysis (WB): 0.2 μg/mL from a representative lot detected Mouse IgG in nonreduced (-) mouse immunoglobulins.

生化／生理作用

This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG. The Fc region of RM104 has been engineered to eliminate the binding of Fc receptor.

外觀

Protein A purified

Format: Purified

Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.

準備報告

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

分析報告

ELISA Analysis: The specificity and reactivity of a representative lot was confirmed by ELISA analyses.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

does not flash

閃點（°C）

does not flash

分析證明 (COA)

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A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.

White Paper: Further considerations of antibody validation and usage.

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