生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
4A4, monoclonal
物種活性
vertebrates
製造商／商標名
Upstate®
技術
ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同型
IgG1
運輸包裝
wet ice
目標翻譯後修改
phosphorylation (pSer)
1 of 4
本產品 | 05-1000 | 16-455 | AB1607 |
|---|---|---|---|
| species reactivity vertebrates | species reactivity vertebrates | species reactivity - | species reactivity - |
| antibody form purified antibody | antibody form purified antibody | antibody form purified antibody | antibody form affinity isolated antibody |
| clone 4A4, monoclonal | clone 4A4, monoclonal | clone 4A4, monoclonal | clone polyclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| isotype IgG1 | isotype IgG1 | isotype IgG1 | isotype - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
一般說明
取决于能检测到的丝氨酸磷酸化蛋白的分子量。
将蛋白质磷酸化鉴定为一种调节机制，起源于1950年代中期Fischer和Krebs的研究，后来使他们获得了1992年的诺贝尔奖。它是调节多种细胞过程的主要机制，包括细胞分裂，蛋白质合成，转录调节和神经传递。任何给定底物的稳态磷酸化均受激酶和磷酸酶的相反活性控制。现在认为，所有真核细胞蛋白中有三分之一被磷酸化，并且所有磷酸化事件中的大多数发生在丝氨酸和苏氨酸残基上（大于95%）。
免疫原
磷酸丝氨酸与KLH偶联
應用
抗磷酸丝氨酸抗体，克隆4A4是用于WB、IF、FC、IH(P)、ELISA的抗磷酸丝氨酸抗体。
研究亚类
常规翻译后修改
常规翻译后修改
研究类别
信号传导
信号传导
生化／生理作用
所有物种中的丝氨酸磷酸化蛋白
外觀
50 µL 含0.05%叠氮化钠和30%甘油的PBS。
形式：纯化
蛋白G-琼脂糖层析
準備報告
自运送之日起在-20°C下保存2年。
为了最大程度地回收产品，需在取下盖子之前将原始样品管进行离心。如果产品意外发生了冻融，可在2-8°C下以13,000 x g离心10分钟。
为了最大程度地回收产品，需在取下盖子之前将原始样品管进行离心。如果产品意外发生了冻融，可在2-8°C下以13,000 x g离心10分钟。
分析報告
通过免疫印迹分析对来自Calyculin A/Okadaic处理的人A431癌细胞的裂解液进行常规评估。
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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