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本產品 | 16-103 | 16-204 | 16-104 |
|---|---|---|---|
| antibody form purified from hybridoma cell culture | antibody form purified antibody | antibody form purified antibody | antibody form - |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone 4G10®, monoclonal | clone 4G10®, monoclonal | clone 4G10®, monoclonal | clone 4G10®, monoclonal |
| isotype IgG2bκ | isotype IgG2bκ | isotype IgG2bκ | isotype - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| technique(s) immunoprecipitation (IP): suitable, western blot: suitable | technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) western blot: suitable | technique(s) immunocytochemistry: suitable |
一般說明
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
應用
Anti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.
外觀
Format: Purified
Purified mouse monoclonal IgG2bκ in buffer containing PBS, pH 7.4. Liquid at 2-8ºC.
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10 (FOX-NY [NS-1 derivative] myeloma x spleen cells).
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10 (FOX-NY [NS-1 derivative] myeloma x spleen cells).
分析報告
Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Routinely evaluated on EGF-treated human A431 carcinoma cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).
Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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