05-807

抗磷酸化CREB（Ser133）抗体，克隆634-2

Name: 抗磷酸化CREB（Ser133）抗体，克隆634-2
Brand: MM

clone 634-2, Upstate^®, from mouse

同義詞:

active transcription factor CREB, cAMP responsive element binding protein 1, cAMP-response element-binding protein-1, transactivator protein

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分類程式碼代碼:

12352203

eCl@ss:

32160702

NACRES:

NA.41

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生物源

mouse

品質等級

100

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

634-2, monoclonal

物種活性

rat, mouse

製造商／商標名

Upstate^®

技術

immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

同型

IgG1κ

NCBI登錄號

NM_134442

UniProt登錄號

P16220

運輸包裝

dry ice

目標翻譯後修改

phosphorylation (pSer133)

基因資訊

human ... CREB1(1385)

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本產品	06-519	05-667	06-519-AF647
Sigma-Aldrich 05-807 抗磷酸化CREB（Ser133）抗体，克隆634-2	Sigma-Aldrich 06-519 抗磷酸化-CREB (Ser133) 抗体	Sigma-Aldrich 05-667 Anti-phospho-CREB (Ser133) Antibody, clone 10E9	Sigma-Aldrich 06-519-AF647 Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor^™647 conjugate
antibody form purified antibody	antibody form purified immunoglobulin	antibody form purified antibody	antibody form affinity isolated antibody
biological source mouse	biological source rabbit	biological source mouse	biological source rabbit
clone 634-2, monoclonal	clone polyclonal	clone 10E9, monoclonal	clone polyclonal
species reactivity rat, mouse	species reactivity mouse, rat, hamster, human	species reactivity canine, rat, mouse, human	species reactivity mouse, rat, human
technique(s) immunocytochemistry: suitable, western blot: suitable, immunofluorescence: suitable	technique(s) electrophoretic mobility shift assay: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable	technique(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable	technique(s) immunofluorescence: suitable
shipped in dry ice	shipped in wet ice	shipped in dry ice	shipped in wet ice

一般說明

43 kDa

CREB是一种βZIP转录因子，可通过cAMP反应元件激活靶基因。CREB能够介导来自多种生理刺激的信号，从而调节多种细胞反应。虽然CREB在许多组织中表达，但它在神经系统中起着重要的调节作用。CREB被认为在促进某些神经元群体的神经元存活，前体增殖，神经突向外生长和神经元分化中起关键作用。另外，CREB信号传导参与几种生物的学习和记忆。CREB能够通过与不同的二聚化伴侣的相互作用选择性激活许多下游基因。CREB通过各种信号传导途径（包括Erk，Ca2+和应激信号传导）在Ser133处磷酸化而被激活。参与Ser133磷酸化CREB的一些激酶是p90RSK，MSK，CaMKIV和MAPKAPK-2。

免疫原

基于含有对应于人CREB残基133的磷酸丝氨酸的氨基酸序列的专有免疫原。

表位：Ser133

應用

使用经ICC、IF、WB验证的抗磷酸化CREB（Ser133）抗体，克隆634-2（小鼠单克隆抗体）来检测磷酸化CREB（Ser133），也称为活性转录因子CREB，cAMP反应元件结合蛋白1。

微珠特异性测定：
该批次的1:500至1:32,000稀释液检测到含有磷酸丝氨酸133的CREB肽。该抗体不能识别免疫荧光/免疫细胞化学相同序列

的非磷酸化肽：
据独立实验室报告，该抗体可使用免疫荧光法检测磷酸化-CREB（Ser133）。

研究子类别
转录因子

研究类别
表观遗传学&核功能

生化／生理作用

基于序列同源性，预期具有广泛的物种交叉反应。

磷酸化-CREB（Ser133）。

外觀

形式：纯化

纯化小鼠IgG1κ，溶于含有0.014 M磷酸盐缓冲液（pH 7.6）、0.175 M NaCl、0.07％叠氮化钠和30％甘油的缓冲液中。在-20ºC为液体形式。

蛋白G纯化

準備報告

自收到之日起在-20°C可稳定保存1年。为了最大程度地回收产品，在取下盖子之前，将原始样品瓶进行离心。

分析報告

对照
人乳腺癌，福司可林和FGF处理的SK-N-MC细胞提取物。

已通过蛋白质印迹对福司可林处理的大鼠PC-12和小鼠3T3/A31细胞的RIPA裂解液进行了评估。

蛋白质印迹分析：
该批次的1:1,000至1:4,000稀释液在福司可林处理的大鼠PC-12和小鼠3T3/A31细胞的RIPA裂解液中检测到磷酸化CREB（Ser 133）（图A）。

其他說明

浓度：请参考批次特异性浓缩物的分析证书。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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ZRB1118

Anti-p-CREB (Ser133) Antibody, clone 1H13 ZooMAb^® Rabbit Monoclonal, recombinant, expressed in HEK 293 cells

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

分析證明 (COA)

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